Rhizosphere bacteria affected by transgenic potatoes with antibacterial activities compared with the effects of soil, wild-type potatoes, vegetation stage and pathogen exposure

FEMS Microbiology Ecology
Frank RascheA Sessitsch

Abstract

A greenhouse experiment was performed to analyze a potential effect of genetically modified potatoes expressing antibacterial compounds (attacin/cecropin, T4 lysozyme) and their nearly isogenic, nontransformed parental wild types on rhizosphere bacterial communities. To compare plant transformation-related variations with commonly accepted impacts caused by altered environmental conditions, potatoes were cultivated under different environmental conditions, for example using contrasting soil types. Further, plants were challenged with the blackleg pathogen Erwinia carotovora ssp. atroseptica. Rhizosphere soil samples were obtained at the stem elongation and early flowering stages. The activities of various extracellular rhizosphere enzymes involved in the C-, P- and N-nutrient cycles were determined as the rates of fluorescence of enzymatically hydrolyzed substrates containing the highly fluorescent compounds 4-methylumbelliferone or 7-amino-4-methyl coumarin. The structural diversity of the bacterial communities was assessed by 16S rRNA-based terminal restriction fragment length polymorphism analysis, and 16S rRNA gene clone libraries were established for the flowering conventional and T4 lysozyme-expressing Desirée lines grown...Continue Reading

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Citations

Oct 18, 2012·Applied Microbiology and Biotechnology·D K Choudhary
Feb 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sandrine DemanèchePascal Simonet
Dec 12, 2012·Applied and Environmental Microbiology·Özgül InceogluJan Dirk van Elsas
Jun 17, 2008·Applied and Environmental Microbiology·Kathryn L OliverWilliam E Hintz
Apr 12, 2012·Applied and Environmental Microbiology·Michael PancherAndrea Campisano
Jun 12, 2013·Microbes and Environments·Nobutaka SomeyaSeishi Ikeda
Mar 3, 2015·Research in Microbiology·Alessandra TurriniManuela Giovannetti
Feb 21, 2014·The ISME Journal·Lucas W MendesSiu M Tsai
Sep 8, 2011·Microbial Ecology·Özgül İnceoğluJan Dirk van Elsas
Jan 6, 2016·Plant Molecular Biology·Andrew LareenPatrick Schäfer
Nov 1, 2008·Microbial Biotechnology·Martin Filion
May 23, 2014·Journal of Applied Microbiology·A K SinghS K Dubey
Apr 1, 2010·Environmental Microbiology Reports·Stéphane UrozFrancis Martin
Aug 12, 2010·FEMS Microbiology Ecology·Nicole WeinertKornelia Smalla
Jan 9, 2016·Journal of Applied Microbiology·K B de Almeida LopesG Degrassi
Nov 22, 2016·Environmental Microbiology·Abdul SamadAngela Sessitsch
Aug 24, 2017·FEMS Microbiology Ecology·Thiago R SchlemperEiko E Kuramae
Apr 17, 2018·Phytopathology·Kamrun NaharSean Whitney
Jun 10, 2010·FEMS Microbiology Ecology·Stéphane CompantAngela Sessitsch
Jan 6, 2011·FEMS Microbiology Ecology·Stéphanie DialloXavier Latour

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