Abstract
We sought to elucidate the role of Rho guanine nucleotide exchange factor 5 (ARHGEF5) in tumorigenesis of lung adenocarcinoma cells. ARHGEF5 protein levels were assessed in 91 human lung adenocarcinoma specimens, and A549 and NCI-H1650 cells, by IHC and Western blotting. In addition, ARHGEF5 mRNA expression was evaluated by quantitative reverse transcriptase-PCR. Furthermore, ARHGEF5 long and short isoform coexpression was detected by immunofluorescence. Finally, flow cytometry; CCK8 and wound-healing assays; cell invasion, migration and adhesion; and xenografts were used to evaluate the biologic significance of ARHGEF5. ARHGEF5 was significantly increased in lung adenocarcinoma tissues and cell lines. Interestingly, ARHGEF5 levels were significantly associated with tumor grade and pathologic stage, but not age, gender, T stage, or lymph node metastasis status. ARHGEF5 knockdown by RNAi resulted in dramatically reduced proliferation, adhesion, invasion, and migratory capability of A549 and NCI-H1650 cells. Likewise, protein levels of p-Src, p-Akt, and NF-κB were significantly decreased after ARHGEF5 knockdown. In parallel, increased S-phase population and MMP-2/cyclin D1 expression were observed in the cancer cells, which were ...Continue Reading
References
Aug 1, 1995·Cancer Genetics and Cytogenetics·S TakaiT Miki
Mar 17, 1997·International Journal of Cancer. Journal International Du Cancer·S B KondapakaK B Reddy
Sep 18, 1997·Journal of the National Cancer Institute·A F Chambers, L M Matrisian
Jun 1, 1997·The International Journal of Biochemistry & Cell Biology·A PardoM Selman
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Nov 24, 1999·Clinical Genetics·S Andrew
Jan 15, 2000·Molecular Cell·B P EliceiriD A Cheresh
Oct 2, 2001·CA: a Cancer Journal for Clinicians·R T GreenleeM Thun
Nov 1, 2001·Respiratory Research·W C Parks, S D Shapiro
Nov 28, 2001·The Journal of Biological Chemistry·Elvira OlasoScott L Friedman
Dec 26, 2001·American Journal of Respiratory Cell and Molecular Biology·Mahito SatoMasahiko Kurabayashi
Mar 15, 2002·Seminars in Oncology·Philip Bonomi
Oct 22, 2002·Biochemical and Biophysical Research Communications·Hyeon G YooYoung D Jung
Jan 16, 2003·Oncogene·Asha NairDevarajan Karunagaran
Jun 27, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T MasakiS Kuriyama
Jul 2, 2003·Oncogene·Lanxi SongEric B Haura
Dec 10, 2003·Human Molecular Genetics·Marie-Anne DebilyDominique Piatier-Tonneau
Mar 5, 2004·Journal of Cell Science·Margaret C Frame
Mar 17, 2004·Annals of Surgery·Mohamed M M Abdel-LatifJohn V Reynolds
Apr 3, 2004·Molecular and Cellular Biology·Atsushi SuzukiHiroyasu Esumi
Aug 6, 2004·Acta Biochimica Et Biophysica Sinica·Min HouYun-Song Zhu
Oct 27, 2004·Apoptosis : an International Journal on Programmed Cell Death·M OsakiH Ito
Dec 17, 2004·Cellular Signalling·Xiaozhen XieToru Miki
Jan 4, 2005·Biochemical Pharmacology·Shiu-Chen HuangJen-Kun Lin
Jan 18, 2005·Cancer Cell·Martin E Fernandez-ZapicoDaniel D Billadeau
May 12, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Pasi A JänneBruce E Johnson
Nov 2, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marta SegarraMaria C Cid
Nov 4, 2005·Cancer Research·Xiaolin ZiBang H Hoang
Jan 9, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jérôme RollinYves Gruel
Oct 2, 2007·Biochemical Pharmacology·Yi-Chia HuangChih-Hsin Tang
Jul 9, 2008·The Journal of Cell Biology·Tsukasa OikawaTadaomi Takenawa
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daizaburo HirataYataro Daigo
Apr 8, 2009·Proceedings of the American Thoracic Society·Alain C BorczukCharles A Powell
Nov 4, 2010·Cellular Signalling·Galit Lazer, Shulamit Katzav
Apr 29, 2011·Journal of Cell Science·Miho KuroiwaMasato Okada
Jul 17, 2012·Current Cancer Drug Targets·Yang QiuWei Guo
Nov 13, 2012·PloS One·Weichen XuDavid S Lawrence
Jan 12, 2013·The American Journal of Pathology·Qian-ze DongEn-Hua Wang
Oct 16, 2013·Oncology Reports·Ping HeKang Yang
Citations
Mar 17, 2016·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Lauren Van Der KraakPhilippe Gros
Sep 13, 2016·Oncogenesis·Y KomiyaM Okada
Feb 18, 2017·American Journal of Obstetrics and Gynecology·Sofia MakievaJane E Norman
Apr 25, 2020·DNA and Cell Biology·Xiang JinHongyu Li
Nov 23, 2017·International Journal of Cancer. Journal International Du Cancer·Yingchang LiWenlin Huang
Jan 27, 2019·Cells·Kwanhyeong KimDaeho Park
Jan 29, 2021·Scientific Reports·Brandon S LaneClark D Wells