Rho kinase and PAI-1 in Bartter's/Gitelman's syndromes: relationship to angiotensin II signaling

Journal of Hypertension
Elisa PagninLorenzo A Calò

Abstract

Angiotensin II (Ang II)-mediated activation of Rho kinase (ROK) is involved in the pathophysiology of hypertension and cardiovascular remodeling. ROK also controls plasminogen activator inhibitor-1 (PAI-1) which promotes vascular fibrosis contributing to atherogenesis. Bartter's and Gitelman's syndromes (BS/GS) are useful models to investigate abnormalities of vascular tone regulation, due to their reduced short- and long-term signaling pathways of Ang II. This study evaluated, using BS/GS as a model, ROK and PAI-1 gene and protein expression and the effect of Ang II co-incubation on ROK and PAI-1 gene and protein expression. We measured ROK and PAI-1 gene and protein expression [reverse transcription-polymerase chain reaction (RT-PCR) and Western blot] in mononuclear cells (PBM) from one BS and eight GS patients. The effect of Ang II on ROK and PAI-1 gene and protein expression was also evaluated and compared with 10 controls. ROK gene and protein expression was reduced in BS/GS [0.47 +/- 0.11 densitometric units (d.u.) versus 0.70 +/- 0.04 d.u., P = 0.0038 and 0.39 +/- 0.07 d.u. versus 0.55 +/- 0.07 d.u., P = 0.0026, respectively]. The basal level of PAI-1 gene and protein expression did not differ (0.40 +/- 0.03 d.u. versus ...Continue Reading

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Citations

Feb 18, 2005·Journal of Hypertension·Lorenzo A CalòAchille C Pessina
Nov 17, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Lorenzo A CalòAndrea Semplicini
Jan 11, 2007·Journal of Hypertension·Lorenzo A Calò, Achille C Pessina
Jun 10, 2011·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Lorenzo A CalòAchille C Pessina
Mar 21, 2006·Clinical and Experimental Hypertension : CHE·L A CalòA C Pessina
Mar 26, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Lorenzo A CalòAchille C Pessina
Mar 12, 2015·High Blood Pressure & Cardiovascular Prevention : the Official Journal of the Italian Society of Hypertension·Verdiana RavarottoLorenzo A Calò
Apr 18, 2014·European Journal of Clinical Investigation·Lorenzo A CalòPaul A Davis
Dec 8, 2011·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Lorenzo A CalòAchille C Pessina
May 7, 2020·Journal of Clinical Medicine·Teresa M SecciaLorenzo A Calò
Apr 14, 2006·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Elisa PagninLorenzo A Calò
Mar 18, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Lorenzo A CalòPaolo Pauletto

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