Abstract
Rho kinase (ROCK) regulates the functions of several target proteins via its kinase activity. Therefore, ROCK activity inhibition may provide new possibilities of controlling the in vitro neuronal differentiation of embryonic stem (ES) cells. When we investigated the effects of the ROCK inhibitor Y-27632 on ES cell differentiation, we found that this inhibitor promoted the differentiation of these cells into neurons. Furthermore, we found that ROCK inhibition may promote the neuronal differentiation of ES cells by activating extracellular signal-regulated kinase (ERK) involved in the ERK signaling pathway. In this study, we investigated the effects of specific inhibitors of several cellular signaling components on the promotion of neuronal differentiation in ES cells to clarify the roles of cellular signaling pathways in the ROCK inhibitor-mediated cell differentiation process. Our results suggest that ERK may be activated via the Ras/Raf/MEK, the PI3K/PKC, or the Cdc42/Rac signaling pathways in the ROCK inhibitor-mediated promotion of neuronal differentiation in ES cells.
References
Aug 23, 1996·The Journal of Biological Chemistry·D J CreedonJ C Lawrence
Aug 8, 1996·Biochimica Et Biophysica Acta·C L Carpenter, L C Cantley
Jul 1, 1998·The Journal of Cell Biology·M HiroseS Narumiya
Apr 5, 2002·The Biochemical Journal·Xiao-Chun BaiShen-Qiu Luo
Aug 9, 2002·Molecular and Cellular Biology·Scott T EblenAndrew D Catling
Apr 1, 2006·The Journal of Biological Chemistry·Loriana CastellaniGermana Falcone
Oct 10, 2006·Development, Growth & Differentiation·Zegui LiLing Wei
Mar 10, 2007·Archivum Immunologiae Et Therapiae Experimentalis·Jianjian Shi, Lei Wei
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jul 3, 2007·Cell·Brendan D Manning, Lewis C Cantley
Sep 29, 2007·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·André SchmandkeStephen M Strittmatter
Feb 20, 2008·Current Opinion in Cell Biology·Michael F Olson
Jul 4, 2008·PloS One·Tomoko NishimuraKenji Hashimoto
Sep 22, 2010·European Journal of Pharmacology·Takahiko MinaseKenji Hashimoto
Jan 8, 2011·Brain Research·Kanako ItohKenji Hashimoto
Jul 20, 2014·Neuroscience Letters·Yu KamishibaharaNorio Shimizu
Citations
Mar 28, 2019·Stem Cells Translational Medicine·Carla B MelloughMajlinda Lako
Nov 24, 2017·Nanoscale·Kisuk YangSeung-Woo Cho
Jan 7, 2021·Oral Diseases·Weiwei ZhangNiu Yumei
Jan 29, 2021·Frontiers in Cell and Developmental Biology·Islam M SaadeldinRamya A Sindi
Dec 3, 2021·Biochemical Society Transactions·Zheng ZhangYi Zheng