PMID: 9548730May 23, 1998Paper

Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly

The Journal of Cell Biology
C ZhongK Burridge

Abstract

Many factors influence the assembly of fibronectin into an insoluble fibrillar extracellular matrix. Previous work demonstrated that one component in serum that promotes the assembly of fibronectin is lysophosphatidic acid (Zhang, Q., W.J. Checovich, D.M. Peters, R.M. Albrecht, and D.F. Mosher. 1994. J. Cell Biol. 127:1447-1459). Here we show that C3 transferase, an inhibitor of the low molecular weight GTP-binding protein Rho, blocks the binding of fibronectin and the 70-kD NH2-terminal fibronectin fragment to cells and blocks the assembly of fibronectin into matrix induced by serum or lysophosphatidic acid. Microinjection of recombinant, constitutively active Rho into quiescent Swiss 3T3 cells promotes fibronectin matrix assembly by the injected cells. Investigating the mechanism by which Rho promotes fibronectin polymerization, we have used C3 to determine whether integrin activation is involved. Under conditions where C3 decreases fibronectin assembly we have only detected small changes in the state of integrin activation. However, several inhibitors of cellular contractility, that differ in their mode of action, inhibit cell binding of fibronectin and the 70-kD NH2-terminal fibronectin fragment, decrease fibronectin incorp...Continue Reading

References

Jun 20, 1978·Annals of the New York Academy of Sciences·M H HeggenessS J Singer
Nov 1, 1978·Cell·R O Hynes, A T Destree
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Oct 1, 1992·Current Opinion in Cell Biology·D F MosherJ A McDonald
May 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T DarribèreJ C Boucaut
Jun 1, 1991·The Journal of Cell Biology·J E Schwarzbauer
Jun 8, 1987·FEBS Letters·M A ChernousovV E Koteliansky
Dec 1, 1986·The Journal of Cell Biology·F Grinnell
Aug 1, 1983·The Journal of Cell Biology·K Burridge, L Connell
Jul 1, 1995·The Journal of Cell Biology·M S KinchK Burridge
Oct 1, 1995·The Journal of Cell Biology·L P Cramer, T J Mitchison
Apr 1, 1995·Current Opinion in Cell Biology·W H Moolenaar
Mar 1, 1995·Experimental Cell Research·N L Halliday, J J Tomasek
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·H P Erickson
Dec 1, 1994·The Journal of Cell Biology·Q ZhangD F Mosher
Nov 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·W J Checovich, D F Mosher
Apr 1, 1996·The Journal of Cell Biology·D C HockingP J McKeown-Longo
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Jul 1, 1996·The Journal of Cell Biology·J L SechlerJ E Schwarzbauer
Oct 1, 1996·Current Biology : CB·A J Ridley
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Jan 17, 1997·The Journal of Biological Chemistry·K C InghamS V Litvinovich

❮ Previous
Next ❯

Citations

Nov 13, 2002·Arthritis and Rheumatism·Eugene Y KissinRobert Lafyatis
Jul 12, 2002·Cell Motility and the Cytoskeleton·Kevin D CostaFrank C-P Yin
Jul 27, 2007·Cell Biochemistry and Biophysics·Shelly R PeytonAndrew J Putnam
Jun 5, 2004·Ageing Research Reviews·J Labat-Robert
Feb 27, 2004·Biochimica Et Biophysica Acta·Jane Sottile
Oct 29, 2003·Current Opinion in Biotechnology·Boris Hinz, Giulio Gabbiani
Jun 8, 2002·Journal of Molecular Biology·Andres F OberhauserJulio M Fernandez
Aug 30, 2003·Journal of Molecular Biology·Klára BriknarováKathryn R Ely
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·T S PanettiD F Mosher
Dec 30, 1999·Biomaterials·K B McClary, D W Grainger
Sep 17, 2002·Current Opinion in Cell Biology·Caroline H Damsky, Dusko Ilić
Nov 18, 1998·Current Opinion in Cell Biology·C G Galbraith, M P Sheetz
Nov 18, 1998·Current Opinion in Cell Biology·N Boudreau, M J Bissell
Oct 6, 1999·Current Opinion in Cell Biology·J E Schwarzbauer, J L Sechler
Oct 6, 1999·Current Opinion in Cell Biology·C Streuli
Apr 21, 1999·Current Opinion in Cell Biology·S M Schoenwaelder, K Burridge
Nov 24, 2001·Trends in Cell Biology·M A Schwartz
Jul 3, 2003·Trends in Cell Biology·Susann Schenk, Vito Quaranta
Aug 1, 2000·Trends in Biochemical Sciences·M A Schwartz, S J Shattil
Dec 10, 2002·Current Opinion in Chemical Biology·Milan Mrksich
Dec 15, 2011·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amber N Stratman, George E Davis
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Viola Vogel, Michael Sheetz
Oct 24, 2009·Nature Reviews. Molecular Cell Biology·Miguel Vicente-ManzanaresAlan Rick Horwitz
Nov 12, 2009·Nature Reviews. Molecular Cell Biology·Patrick T CaswellJim C Norman
Apr 22, 2011·Nature Reviews. Molecular Cell Biology·Christopher C DuFortValerie M Weaver
Sep 5, 2002·British Journal of Pharmacology·Chrystelle Cario-ToumaniantzGervaise Loirand
Dec 23, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kristopher E KubowViola Vogel
Sep 11, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Wesley R LegantViola Vogel
Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Enrico KlotzschViola Vogel
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lizhi CaoThomas H Barker
Oct 7, 2010·The Journal of Biological Chemistry·Ran YouPaula J McKeown-Longo
Mar 9, 2012·The Journal of Biological Chemistry·Keisuke ItagakiFumio Fukai
Dec 10, 2008·The Journal of Biological Chemistry·Nancy W KaruriJean E Schwarzbauer
Jun 5, 2010·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Jonathan StrickerMargaret L Gardel
Jul 14, 2011·Physical Biology·A SzabóA Czirók
Nov 26, 2010·Stem Cells and Development·Stephanie Piecewicz, Shiladitya Sengupta
May 24, 2007·Tissue Engineering·Marian PereiraPrabhas V Moghe

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