Rho-Signaling-Directed YAP/TAZ Activity Underlies the Long-Term Survival and Expansion of Human Embryonic Stem Cells.

Cell Stem Cell
Masatoshi OhgushiYoshiki Sasai

Abstract

Human embryonic stem cells (hESCs) can survive and proliferate for an extended period of time in culture, but unlike that of tumor-derived cells, this form of cellular immortality does not depend on genomic aberrations. In this study, we sought to elucidate the molecular basis of this long-term growth property of hESCs. We found that the survival of hESCs depends on the small GTPase Rho and its activator AKAP-Lbc. We show that AKAP-Lbc/Rho signaling sustains the nuclear function of the transcriptional cofactors YAP and TAZ by modulating actin microfilament organization. By inducing reprogramming and differentiation, we found that dependency on this Rho signaling pathway is associated with the pluripotent state. Thus, our findings show that the capacity of hESCs to undergo long-term expansion in vitro is intrinsically coupled to their cellular identity through interconnected molecular circuits that link cell survival to pluripotency.

References

Apr 21, 1995·The Journal of Biological Chemistry·Y ZhengD Toksoz
Jan 7, 1998·Oncogene·M F OlsonA Hall
Apr 3, 2003·Nature Reviews. Cancer·Clemens A Schmitt
May 30, 2003·Nature·Kazutoshi TakahashiShinya Yamanaka
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Kent L RossmanJohn Sondek
Feb 8, 2006·European Journal of Cell Biology·Dario DivianiAline Appert-Collin
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jun 2, 2007·Cell·Johannes L BosAlfred Wittinghofer
Jun 24, 2008·Nature Cell Biology·Xaralabos VarelasJeffrey L Wrana
Jun 27, 2008·Genes & Development·Bin ZhaoKun-Liang Guan
Feb 9, 2010·The Journal of Biological Chemistry·Chantal M MayersJames H Segars
Mar 26, 2010·Nature·Michal ZalzmanMinoru S H Ko
Apr 29, 2011·Development·Beatriz García FernándezFlorence Janody
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Aug 13, 2011·Development·Ken-Ichi WadaHiroshi Sasaki
Jan 31, 2012·Human Molecular Genetics·Han QinMiguel Ramalho-Santos
Aug 3, 2012·Cold Spring Harbor Perspectives in Biology·Jennifer Nichols, Austin Smith
Oct 5, 2012·Seminars in Cell & Developmental Biology·Annie M Tremblay, Fernando D Camargo
Feb 23, 2013·Genes & Development·Fa-Xing Yu, Kun-Liang Guan
Jun 5, 2013·The Journal of Cell Biology·Eliana P LucasBarry J Thompson
Dec 24, 2013·Nature Cell Biology·Morvarid MohseniFernando D Camargo
Mar 18, 2015·Nature·Sean PorazinskiMakoto Furutani-Seiki

❮ Previous
Next ❯

Citations

Feb 11, 2016·Nature Reviews. Molecular Cell Biology·Leehee WeinbergerJacob H Hanna
Oct 28, 2015·The Journal of Cell Biology·Evan Heller, Elaine Fuchs
Jan 27, 2017·Small GTPases·Arie HorowitzLorraine Iacovitti
Apr 11, 2017·Molecular and Cellular Endocrinology·An Yu, Weiwei Dang
Feb 20, 2018·Tissue Engineering. Part B, Reviews·Sarita KumariDeepa Subramanyam
Sep 28, 2017·Nature Reviews. Molecular Cell Biology·Tito PancieraStefano Piccolo
May 12, 2018·FEBS Letters·Shuh Narumiya, Dean Thumkeo
Aug 24, 2017·Annals of the New York Academy of Sciences·Varun Arvind, Alice H Huang
May 12, 2018·Journal of Tissue Engineering and Regenerative Medicine·Liqing SongYan Li
Sep 22, 2018·The Journal of Clinical Endocrinology and Metabolism·Sinnie Sin Man NgJames H Segars
Jan 13, 2019·The Journal of Biological Chemistry·Jaemin ByunJunichi Sadoshima
May 21, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alexandra StanleyEileen M Shore
Jul 28, 2016·Science Signaling·Junning YangArie Horowitz
Nov 27, 2019·Cellular and Molecular Neurobiology·Taohui OuyangNa Zhang
Apr 2, 2020·Cells·Daniel J OwensCatherine Coirault
Dec 31, 2016·Oncotarget·June Sung BaeHo Lee
Jan 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Suping ZhangThomas J Kipps
Jun 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ameer L ElaimyArthur M Mercurio
Aug 23, 2020·Cells·Sepideh Fallah, Jean-François Beaulieu
Aug 5, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takashi IkedaShinji Masui
Aug 14, 2018·Clinical and Translational Medicine·Oleg DobrokhotovHiroaki Hirata

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Stem Cells International
Fuliang DuPerng-Chih Shen
Seikagaku. The Journal of Japanese Biochemical Society
Shinji Masui
Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
O F GordeevaN G Khrushchov
© 2021 Meta ULC. All rights reserved