Rho4 interaction with exocyst and septins regulates cell separation in fission yeast

Microbiology
Pilar PerezBeatriz Santos

Abstract

Rho GTPases are small proteins present in all eukaryotic cells, from yeast to mammals, with a function in actin organization and morphogenetic processes. Schizosaccharomyces pombe Rho4 is not essential but it displays a role during cell separation at high temperature. In fact, Rho4 is involved in the secretion of the hydrolytic enzymes that are required for cell septum degradation during this process. In rho4Δ cells, vesicles accumulate in the septum area and the glucanases Eng1 and Agn1 are not secreted to the culture medium. The localization of Eng1 and Agn1 depends on the exocyst and the septins. The exocyst is a conserved multiprotein complex important for the targeting and fusion of Golgi-derived vesicles with the plasma membrane. Septins are a family of GTP-binding proteins conserved in eukaryotes that function during cytokinesis. Here we show that Rho4 is required for the proper localization of the exocyst and septins at high temperature. Moreover, pull-down experiments demonstrate that Rho4 can interact with exocyst subunits, such as Sec8 and Exo70, and septin proteins, such as Spn3. We observe that Sec8 preferentially binds to activated GTP-Rho4, suggesting that Sec8 could be an effector of this GTPase. We propose that...Continue Reading

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Citations

Mar 5, 2016·World Journal of Microbiology & Biotechnology·Rebeca Martín-García, Beatriz Santos
May 6, 2016·Frontiers in Cell and Developmental Biology·Magdalena Martin-UrdirozIsabelle Jourdain
May 8, 2016·Cellular Microbiology·Pilar PérezJuan C Ribas
Nov 2, 2019·Communicative & Integrative Biology·Brian Hercyk, Maitreyi Das
Apr 17, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Pilar PérezJose Cansado
May 21, 2020·Molecular Biology of the Cell·Kenneth S GerienJian-Qiu Wu
Nov 19, 2018·Biophysics Reviews·Kenneth S Gerien, Jian-Qiu Wu
Jul 3, 2021·Cells·Jero Vicente-SolerMarisa Madrid

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