RhoA enhances store-operated Ca2+ entry and intestinal epithelial restitution by interacting with TRPC1 after wounding

American Journal of Physiology. Gastrointestinal and Liver Physiology
Hee Kyoung ChungJaladanki N Rao

Abstract

Early mucosal restitution occurs as a consequence of epithelial cell migration to resealing of superficial wounds after injury. Our previous studies show that canonical transient receptor potential-1 (TRPC1) functions as a store-operated Ca(2+) channel (SOC) in intestinal epithelial cells (IECs) and plays an important role in early epithelial restitution by increasing Ca(2+) influx. Here we further reported that RhoA, a small GTP-binding protein, interacts with and regulates TRPC1, thus enhancing SOC-mediated Ca(2+) entry (SOCE) and epithelial restitution after wounding. RhoA physically associated with TRPC1 and formed the RhoA/TRPC1 complexes, and this interaction increased in stable TRPC1-transfected IEC-6 cells (IEC-TRPC1). Inactivation of RhoA by treating IEC-TRPC1 cells with exoenzyme C3 transferase (C3) or ectopic expression of dominant negative RhoA (DNMRhoA) reduced RhoA/TRPC1 complexes and inhibited Ca(2+) influx after store depletion, which was paralleled by an inhibition of cell migration over the wounded area. In contrast, ectopic expression of wild-type (WT)-RhoA increased the levels of RhoA/TRPC1 complexes, induced Ca(2+) influx through activation of SOCE, and promoted cell migration after wounding. TRPC1 silencin...Continue Reading

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Citations

Nov 6, 2018·Human Genetics·Ekaterina OrlovaMichael J Morowitz
Jan 17, 2019·American Journal of Physiology. Cell Physiology·Li-Ping JiangJaladanki N Rao
Dec 2, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Navneeta RathorJaladanki N Rao
Jan 20, 2017·American Journal of Physiology. Cell Physiology·Peng-Yuan WangJaladanki N Rao
Oct 27, 2017·American Journal of Physiology. Cell Physiology·Lindsay M HerbertNikki L Jernigan
Nov 3, 2020·Frontiers in Pharmacology·Giorgia ChinigòDimitra Gkika
May 18, 2021·Cells & Development·Brenda Canales Coutiño, Roberto Mayor
Jun 29, 2021·Frontiers in Oncology·María Paz SaldíasOscar Cerda
Aug 31, 2021·Cells & Development·Brenda Canales Coutiño, Roberto Mayor

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