RhoA is activated during respiratory syncytial virus infection

Virology
T L GowerBarney S Graham

Abstract

Respiratory syncytial virus (RSV) is an important human pathogen that can cause severe and life-threatening respiratory infections in infants and immunocompromised adults. We have recently shown the RSV F glycoprotein, which mediates viral fusion and entry, interacts with the cellular protein RhoA in two-hybrid and in vitro binding assays. Whether this interaction occurs in living cells remains an open question. However, because RhoA signaling is associated with many cellular functions relevant to RSV pathogenesis such as actin cytoskeleton organization, expression of proinflammatory cytokines, and smooth muscle contraction, we asked whether RhoA activation occurred during RSV infection of HEp-2 cells. We found that the amount of isoprenylated and membrane-bound RhoA in RSV-infected cultures was increased. Further evidence of RhoA activation was demonstrated by downstream signaling activity mediated by RhoA. There was an increase in p130(cas) phosphorylation during RSV infection, which was prevented by Y-27632, a specific inhibitor of Rho kinase, or lovastatin, an HMG-CoA reductase inhibitor that reduces the synthesis of groups needed for isoprenylation. In addition, RSV infection of HEp-2 cells resulted in an increase in the f...Continue Reading

References

May 1, 1978·The Journal of General Virology·D L Tyrrell, E Norrby
Jan 1, 1987·Membrane Biochemistry·D P Sarkar, R Blumenthal
Oct 1, 1988·Journal of Medical Virology·B S GrahamD T Karzon
Jan 1, 1987·AIDS Research and Human Retroviruses·F Gonzalez-ScaranoJ A Hoxie
May 1, 1995·Journal of Virology·H B BernsteinE Hunter
Nov 1, 1993·Trends in Microbiology·J M Bergelson, R W Finberg
Aug 1, 1996·Journal of Biochemistry·S Narumiya
Aug 1, 1997·Trends in Microbiology·D Falke
Feb 7, 1998·Science·A Hall
Jun 19, 1998·Current Opinion in Structural Biology·S J Gamblin, S J Smerdon
Aug 26, 1998·Molecular and Cellular Biology·J H ChangS J Burakoff
Jun 8, 1999·The Journal of Clinical Investigation·R SinghJ R Sedor
Apr 18, 2000·The Journal of General Virology·Q Yao, R W Compans
Sep 12, 2000·Journal of Virology·G Y Lin, R A Lamb

❮ Previous
Next ❯

Citations

Nov 26, 2009·Cellular and Molecular Life Sciences : CMLS·Nadezhda TikhmyanovaErica A Golemis
Jan 25, 2008·Journal of Virology·Julia Eckardt-MichelGert Zimmer
Jan 15, 2003·Journal of Virology·Lewis H McCurdy, Barney S Graham
Mar 25, 2006·Uirusu·Masato Tsurudome
Feb 28, 2002·Trends in Immunology·James HarrisChris J Howard
Apr 3, 2014·Small GTPases·Céline Van den BroekeHerman W Favoreel
Sep 10, 2002·The Journal of Infection·D Hacking, J Hull
Sep 28, 2016·Virology Journal·Shadi ShahriariReena Ghildyal
Oct 1, 2007·Therapeutic Advances in Respiratory Disease·Lynette B FernandesRoy G Goldie
Jan 18, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yuh-Chin T HuangJoleen M Soukup
Feb 1, 2017·Current Clinical Microbiology Reports·Hector C AguilarGunner P Johnston
Dec 23, 2020·International Journal of Molecular Sciences·Héloïse LeroySerge Benichou
May 20, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Debra T LinfieldFariba Rezaee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.