RhoA/ROCK Signaling Pathway Mediates Shuanghuanglian Injection-Induced Pseudo-allergic Reactions

Frontiers in Pharmacology
Jia-yin HanAi-hua Liang

Abstract

Background: Shuanghuanglian injection (SHLI) is a famous Chinese medicine used as an intravenous preparation for the treatment of acute respiratory tract infections. In the recent years, the immediate hypersensitivity reactions induced by SHLI have attracted broad attention. However, the mechanism involved in these reactions has not yet been elucidated. The present study aims to explore the characteristics of the immediate hypersensitivity reactions induced by SHLI and deciphers the role of the RhoA/ROCK signaling pathway in these reactions.Methods:SHLI-immunized mice or naive mice were intravenously injected (i.v.) with SHLI (600 mg/kg) once, and vascular leakage in the ears was evaluated. Passive cutaneous anaphylaxis test was conducted using sera collected from SHLI-immunized mice. Naive mice were administered (i.v.) with a single dose of 150, 300, or 600 mg/kg of SHLI, and vascular leakage, histamine release, and histopathological alterations in the ears, lungs, and intestines were tested.In vitro, human umbilical vein endothelial cell (HUVEC) monolayer was incubated with SHLI (0.05, 0.1, or 0.15 mg/mL), and the changes in endothelial permeability and cytoskeleton were observed. Western blot analysis was performed and ROCK ...Continue Reading

References

Feb 7, 1998·Science·A Hall
Apr 21, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J RingR Engst
Aug 19, 2000·Circulation Research·G P van Nieuw AmerongenV W van Hinsbergh
Feb 17, 2001·Journal of Applied Toxicology : JAT·K H DiehlUNKNOWN European Federation of Pharmaceutical Industries Association and European Centre for the Validation of Alternative Methods
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Kirsi Riento, Anne J Ridley
Oct 21, 1961·Nature·S O FREEDMANA H SEHON
Mar 1, 1964·The Journal of Allergy·S O FREEDMAN
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mien V HoangDonald R Senger
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Aron B Jaffe, Alan Hall
Sep 1, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·G P van Nieuw AmerongenV W M van Hinsbergh
Sep 15, 2007·Biochimica Et Biophysica Acta·Andrea Hartsock, W James Nelson
Oct 12, 2007·Journal of Cell Science·Stéphanie Pellegrin, Harry Mellor
Oct 27, 2007·Biochimica Et Biophysica Acta·Yann Wallez, Philippe Huber
Jul 5, 2008·EMBO Reports·Daniel NyqvistElisabetta Dejana
Jul 24, 2008·Journal of Thrombosis and Haemostasis : JTH·A AghajanianK Burridge
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Phyllus Y Mong, Qin Wang
Jun 29, 2010·Acta Pharmacologica Sinica·Fang-hua HuangAi-jun Chen
Feb 26, 2011·Journal of Evidence-based Medicine·Li WangYouping Li
Apr 13, 2011·British Journal of Clinical Pharmacology·Derek G Waller
Jun 29, 2011·Journal of Separation Science·Lijuan LiuQingbo Zhang
Dec 15, 2011·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Richard Warrington, Fanny Silviu-Dan
Apr 3, 2012·Drug Discoveries & Therapeutics·H WangZ P Liu
May 1, 2012·Journal of Cell Science·Sari TojkanderPekka Lappalainen
Jul 27, 2012·International Archives of Allergy and Immunology·Kevin FarnamM Eric Gershwin
Oct 23, 2012·International Journal of Toxicology·Mingbao LinXiaohui Fan
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Mingbao LinXiaohui Fan
Sep 21, 2013·Developmental Cell·Monica GiannottaElisabetta Dejana
Nov 7, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yuan GaoYun Qi
Nov 12, 2013·Journal of Pharmaceutical and Biomedical Analysis·Shengli HanLangchong He
Apr 5, 2014·Allergy·P DemolyB Y- H Thong
Jun 14, 2014·Chemical Immunology and Allergy·Johannes RingKarl-Christian Bergmann
Apr 11, 2015·Nature Communications·Constantinos M MikelisJ Silvio Gutkind
May 17, 2015·Journal of Ethnopharmacology·Fang WangGuoping Peng
Jul 16, 2015·Journal of Pharmaceutical and Biomedical Analysis·Hongyang SunShizhong Chen
Aug 16, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Natalia Reglero-RealSussan Nourshargh
Dec 14, 2016·International Archives of Allergy and Immunology·Werner J Pichler, Oliver Hausmann

❮ Previous
Next ❯

Citations

Oct 12, 2019·International Journal of Molecular Sciences·Chen PanAi-Hua Liang
Dec 26, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Shiqi ChenHongcai Shang
Jul 10, 2021·World Journal of Gastroenterology : WJG·Qiu-Ju HanJian Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
pull down
PCA
biopsies
Fluorescence

Software Mentioned

BSW
Cell Sens Standard
DP2

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.