RhoB is regulated by hypoxia and modulates metastasis in breast cancer.

Cancer Reports
Julia A JuDaniele M Gilkes

Abstract

RhoB is a Rho family GTPase that is highly homologous to RhoA and RhoC. RhoA and RhoC have been shown to promote tumor progression in many cancer types; however, a distinct role for RhoB in cancer has not been delineated. Additionally, several well-characterized studies have shown that small GTPases such as RhoA, Rac1, and Cdc42 are induced in vitro under hypoxia, but whether and how hypoxia regulates RhoB in breast cancer remains elusive. To determine whether and how hypoxia regulates RhoB expression and to understand the role of RhoB in breast cancer metastasis. We investigated the effects of hypoxia on the expression and activation of RhoB using real-time quantitative polymerase chain reaction and western blotting. We also examined the significance of both decreased and increased RhoB expression in breast cancer using CRISPR depletion of RhoB or a vector overexpressing RhoB in 3D in vitro migration models and in an in vivo mouse model. We found that hypoxia significantly upregulated RhoB mRNA and protein expression resulting in increased levels of activated RhoB. Both loss of RhoB and gain of RhoB expression led to reduced migration in a 3D collagen matrix and invasion within a multicellular 3D spheroid. We showed that neith...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·P AdamsonA Hall
May 18, 1999·International Journal of Cancer. Journal International Du Cancer·G FritzB Kaina
Mar 22, 2002·Nature Reviews. Cancer·G C Prendergast
Aug 27, 2003·The Journal of Biological Chemistry·Lei WangYi Zheng
Apr 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julien MazieresGilles Favre
Jul 1, 2004·Journal of Cell Science·Matthew WherlockHarry Mellor
Jul 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takao KamaiKen-Ichiro Yoshida
May 14, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Teresa Gómez del PulgarJuan Carlos Lacal
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Aron B Jaffe, Alan Hall
Jan 6, 2006·International Journal of Cancer. Journal International Du Cancer·Yan XueDaiming Fan
Jun 30, 2006·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·X R LiK Y Yang
Nov 11, 2006·International Journal of Cancer. Journal International Du Cancer·Naohito SatoYoshitaka Sekido
Oct 24, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Minzhou HuangLisa D Laury-Kleintop
Oct 25, 2007·Cellular and Molecular Life Sciences : CMLS·K LundgrenG Landberg
Jan 24, 2008·Oncogene·R M BaldwinI A J Lorimer
Mar 15, 2008·Cancer Gene Therapy·B CoudercG Favre
May 8, 2008·FEBS Letters·Francisco M Vega, Anne J Ridley
Mar 31, 2009·Biochimica Et Biophysica Acta·R KarlssonCord Brakebusch
Sep 9, 2010·Cell Communication and Signaling : CCS·Matteo Parri, Paola Chiarugi
Mar 9, 2012·Trends in Pharmacological Sciences·Gregg L Semenza
Apr 28, 2012·Circulation Research·Beata Wojciak-StothardMartin R Wilkins
Mar 16, 2013·Journal of Microscopy·A J Ridley
Sep 28, 2013·Molecular Cancer Therapeutics·Hemant K BidPeter J Houghton
Oct 3, 2013·PloS One·Jeffrey KroonMar Fernandez-Borja
Oct 26, 2013·Future Oncology·Daniele M Gilkes, Gregg L Semenza
Dec 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Daniele M GilkesGregg L Semenza
May 16, 2014·Nature Reviews. Cancer·Daniele M GilkesDenis Wirtz
Jul 31, 2014·Nature Methods·Neville E SanjanaFeng Zhang
Sep 14, 2014·Neuro-oncology·Yufang MaJialiang Wang
Sep 22, 2015·Cellular & Molecular Immunology·Gaoxiang HuangJian Lu
Oct 21, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Changhwan YoonSam S Yoon
Nov 4, 2015·Oncotarget·Angela M Jimenez ValenciaDenis Wirtz
Oct 6, 2016·International Journal of Molecular Sciences·Daniele M Gilkes
Nov 3, 2016·Breast Cancer Research and Treatment·András LánczkyBalázs Győrffy
Feb 19, 2017·Molecular Cancer Research : MCR·Julia A JuDaniele M Gilkes

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Related Papers

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Julien MazièresGilles Favre
Histology and Histopathology
Minzhou Huang, G C Prendergast
© 2021 Meta ULC. All rights reserved