Abstract
The T cell activation pathway involves an increase in mitochondrial activity. This can be evaluated in individual cells using the fluorescent probe rhodamine 123 (Rh123) and flow cytometry. Peripheral blood mononuclear cells (PBMC) were stimulated with optimal concentrations of phytohaemagglutinin (PHA), superantigens (Sag) SEA and SEC2, and allogeneic cells. Activation kinetics were followed at days 1, 2, 4 and 7. In all activation conditions, Rh123 uptake was augmented with the CD25 expression, cell size, and DNA synthesis. Rh123 uptake reflected an increase in mitochondrial activity and mass, as assessed by experiments in which Rh123 was substituted for by the 10-nonyl acridine orange, which stains mitochondria in an energy-independent manner. The spectral characteristics of Rh123 allowed us to double stain cells with Rh123 and phycoerythrin-conjugated monoclonal antibodies. In PHA-activated cultures, CD4+ and CD8+ cells incorporated essentially the same amount of Rh123 at all time points, suggesting that the two subsets did not differ in their activation kinetics. Accordingly, after 1 week of culture, no significant modification in the CD4/CD8 ratio was observed. Sag-activated CD4+ cells incorporated a higher amount of Rh12...Continue Reading
References
Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Aug 1, 1992·European Journal of Immunology·R NisiniR D'Amelio
Apr 1, 1992·Scandinavian Journal of Immunology·R BiselliA Fattorossi
Jan 1, 1991·Cytometry·E F SrourR Hoffman
May 1, 1988·Cytometry·M H RatinaudR Julien
Feb 1, 1989·Immunological Reviews·C A JanewayS Buxser
May 1, 1986·Cytometry·L BenelJ C Mounolou
Jul 1, 1985·Cellular Immunology·M KoponenF Loor
Nov 1, 1980·Cytometry·J T ThornthwaiteW J Temple
Jul 1, 1982·Cancer·A W ClarkS K Wilson
Jan 1, 1982·Cell·L V JohnsonL B Chen
Jan 1, 1983·The Journal of Cell Biology·J M Collins, K A Foster
Jan 15, 1984·The Biochemical Journal·P ArslanT Pozzan
Mar 1, 1981·The Journal of Cell Biology·L V JohnsonL B Chen
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·L B ChenT J Lampidis
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L V JohnsonL B Chen
May 1, 1981·The Journal of Cell Biology·T W James, R Bohman
Apr 1, 1994·AIDS·R NisiniR D'Amelio
Apr 21, 1994·Nature·T S JardetzkyD C Wiley
Jun 3, 1994·Journal of Immunological Methods·W G TelfordP J Fraker
Jan 1, 1993·Immunogenetics·P ForteA Fantoni
Feb 1, 1993·Seminars in Immunology·N R Gascoigne
Citations
Feb 24, 2000·Cytometry·J F KeijJ A Steinkamp
Jun 9, 1998·Journal of Cellular Physiology·J P Freyer
Jun 1, 1997·International Journal of Immunopharmacology·M VioraA Fattorossi
Dec 15, 2007·Nature Protocols·Cristiano Ferlini, Giovanni Scambia
Sep 30, 1998·British Journal of Haematology·L PierelliG Leone
Oct 29, 2000·European Journal of Biochemistry·B D FollstadG Stephanopoulos
Jul 1, 1997·European Journal of Biochemistry·J ZhangJ Sjövall
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·M HothR S Lewis
Sep 13, 2011·Neurochemical Research·D Alonso-AlconadaE Hilario
Aug 1, 1997·European Journal of Immunology·Y XueM Jondal
May 1, 2004·Environmental Toxicology and Pharmacology·Francesco TomeiEnrico Tomao
Mar 15, 1997·Analytical Biochemistry·D KunzW S Kunz
Nov 15, 2006·Neuroscience·M AkbariH E Krokan
Feb 27, 1999·Experimental Cell Research·C FerliniA Fattorossi
Oct 25, 2017·Parasite Immunology·K Donskow-ŁysoniewskaM Doligalska
Sep 1, 2006·Current Protocols in Toxicology·Stacey E AndersonBarbara J Meade
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A PazirandehS Okret
Jan 9, 2021·Journal of Cellular Biochemistry·Wojciech TrybusGrzegorz Król
Mar 7, 2021·Plants·Taras PasternakSerhii Kondratenko