Rhodanese incorporated in Langmuir and Langmuir-Blodgett films of dimyristoylphosphatidic acid: Physical chemical properties and improvement of the enzyme activity

Colloids and Surfaces. B, Biointerfaces
Felipe Tejada de Araújo, Luciano Caseli

Abstract

Preserving the catalytic activity of enzymes immobilized in bioelectronics devices is essential for optimal performance in biosensors. Therefore, ultrathin films in which the architecture can be controlled at the molecular level are of interest. In this work, the enzyme rhodanese was adsorbed onto Langmuir monolayers of the phospholipid dimyristoylphosphatidic acid and characterized by surface pressure-area isotherms, polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS), and Brewster angle microscopy (BAM). The incorporation of the enzyme (5% in mol) in the lipid monolayer expanded the film, providing small surface domains, as visualized by BAM. Also, amide bands could be identified in the PM-IRRAS spectra, confirming the presence of the enzyme at the air-water interface. Structuring of the enzyme into α-helices was identified in the mixed monolayer and was preserved when the film was transferred from the liquid interface to solids supports as Langmuir-Blodgett (LB) films. The enzyme-lipid LB films were then characterized by fluorescence spectroscopy, PM-IRRAS, and atomic force microscopy. Measurements of the catalytic activity towards cyanide showed that the enzyme accommodated in the LB films preserve...Continue Reading

References

Jul 15, 2003·Biomacromolecules·Sarita V MelloJoseph J DeFrank
Sep 27, 2005·Advances in Colloid and Interface Science·Agnès P Girard-EgrotLoïc J Blum
Sep 20, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Luciano CaseliOsvaldo N Oliveira
Aug 31, 2011·Journal of Colloid and Interface Science·Nathaly C M ZanonLuciano Caseli
Jan 13, 2015·Colloids and Surfaces. B, Biointerfaces·Fabio Antonio Scholl, Luciano Caseli

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Citations

Nov 5, 2019·Journal of Colloid and Interface Science·Rafael Leonardo Cruz Gomes da SilvaRoger M Leblanc
Apr 25, 2020·Colloids and Surfaces. B, Biointerfaces·Luiz Henrique Rodrigues Rola PossarleLuciano Caseli
Apr 14, 2021·The Science of the Total Environment·Minglan XuLin Du
May 16, 2017·Bioconjugate Chemistry·Ahmet Ulu, Burhan Ates
Feb 6, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Raul T RodriguesLuciano Caseli
May 14, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Felipe Tejada AraujoLuciano Caseli

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