Abstract
Lipophorin is a major lipoprotein that transports lipids in insects. In Rhodnius prolixus, it transports lipids from midgut and fat body to the oocytes. Analysis by thin-layer chromatography and densitometry identified the major lipid classes present in the lipoprotein as diacylglycerol, hydrocarbons, cholesterol, and phospholipids (PLs), mainly phosphatidylethanolamine and phosphatidylcholine. The effect of preincubation at elevated temperatures on lipophorin capacity to deliver or receive lipids was studied. Transfer of PLs to the ovaries was only inhibited after preincubation of lipophorin at temperatures higher than 55 °C. When it was pretreated at 75 °C, maximal inhibition of phospholipid transfer was observed after 3-min heating and no difference was observed after longer times, up to 60 min. The same activity was also obtained when lipophorin was heated for 20 min at 75 °C at protein concentrations from 0.2 to 10 mg/ml. After preincubation at 55 °C, the same rate of lipophorin loading with PLs at the fat body was still present, and 30% of the activity was observed at 75 °C. The effect of temperature on lipophorin was also analyzed by turbidity and intrinsic fluorescence determinations. Turbidity of a lipophorin solution ...Continue Reading
References
Jan 1, 1992·Archives of Insect Biochemistry and Physiology·K C GondimH Masuda
Jan 1, 1987·Methods in Enzymology·J Horwitz, R L Perlman
May 7, 1974·Biochemistry·L de Meis, H Masuda
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1994·Advances in Protein Chemistry·J L Soulages, M A Wells
Jan 1, 1997·Archives of Insect Biochemistry and Physiology·H S CoelhoH Masuda
Sep 16, 1997·Biochemistry·C Maya-MonteiroS T Ferreira
May 26, 2001·Annual Review of Nutrition·L E CanavosoM A Wells
Jan 1, 1992·Archives of Insect Biochemistry and Physiology·G C AtellaH Masuda
Jan 1, 1995·Archives of Insect Biochemistry and Physiology·G C AtellaH Masuda
Jan 1, 1996·Archives of Insect Biochemistry and Physiology·E A MachadoH Masuda
Mar 26, 2003·Insect Biochemistry and Molecular Biology·L A M GrilloK C Gondim
May 29, 2003·Journal of Insect Physiology·V SevalaC Schal
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jul 6, 2004·Journal of Insect Physiology·Yongliang FanAnne-Geneviève Bagnères
May 3, 2006·Insect Biochemistry and Molecular Biology·Georgia C AtellaMohammed Shahabuddin
Aug 26, 2006·Archives of Insect Biochemistry and Physiology·Giselle A OliveiraGeorgia C Atella
May 2, 2007·Biochemistry·Madhumita GuhaOlga Gursky
Nov 1, 1967·Lipids·J E Storry, B Tuckley
Mar 12, 2008·The FEBS Journal·Sigrid D RoosendaalDick J Van der Horst
Dec 4, 2008·Insect Biochemistry and Molecular Biology·Sigrid D RoosendaalKees W Rodenburg
Dec 17, 2008·Current Protein & Peptide Science·Francisco N BarreraJosé L Neira
Jan 9, 2009·Molecular and Cellular Biochemistry·Dick J Van der HorstKees W Rodenburg
Jun 10, 2009·Insect Biochemistry and Molecular Biology·Leonardo L FrutteroLilián E Canavoso
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·José Rodrigues Coura, João Carlos Pinto Dias
Feb 11, 2011·Archives of Insect Biochemistry and Physiology·Rachel SantosKatia C Gondim