Rhodobacter sphaeroides LexA has dual activity: optimising and repressing recA gene transcription

Nucleic Acids Research
Angels TapiasJordi Barbé

Abstract

Transcription of the Rhodobacter sphaeroides recA promoter (P(recA)) is induced upon DNA damage in a lexA-dependent manner. In vivo experiments demonstrate that LexA protein represses and might also activate transcription of P(recA). Purified R.sphaeroides LexA protein specifically binds the SOS boxes located within the P(recA) region. In vitro transcription analysis, using Escherichia coli RNA polymerase (RNAP), indicated that the presence of LexA may stimulate and repress transcription of P(recA). EMSA and DNase I footprinting experiments show that LexA and RNAP can bind simultaneously to P(recA). At low LexA concentrations it enhances RNAP binding to P(recA), stimulates open complex formation and strand separation beyond the transcription start site. At high LexA concentrations, however, RNAP-promoted strand separation is not observed beyond the +5 region. LexA might repress transcription by interfering with the clearance process instead of blocking the access of RNAP to the promoter region. Based on these findings we propose that the R.sphaeroides LexA protein performs fine tuning of the SOS response, which might provide a physiological advantage by enhancing transcription of SOS genes and delaying full activation of the re...Continue Reading

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Citations

Aug 12, 2009·Journal of Bacteriology·Harvey H Kimsey, Matthew K Waldor
Dec 18, 2007·Journal of Bacteriology·Raquel Paes da RochaRodrigo S Galhardo
Oct 15, 2013·Journal of Bacteriology·Jesús ArandaGermán Bou
Mar 14, 2016·Trends in Microbiology·Nadine FornelosMatej Butala
Sep 22, 2007·FEMS Microbiology Reviews·Ivan ErillJordi Barbé
May 20, 2015·Journal of Bacteriology·Neus Sanchez-AlberolaIvan Erill
Nov 6, 2004·Microbiology·Gerard MazónJordi Barbé
Mar 11, 2005·Microbiology·Hannes NahrstedtFriedhelm Meinhardt
Oct 28, 2019·Nature Communications·Katy PoncinXavier De Bolle
Nov 8, 2019·Journal of Biotechnology·Maricruz FernandezSonia Fischer
Oct 7, 2021·Nucleic Acids Research·Miquel Sánchez-OsunaIvan Erill

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