PMID: 11914155Mar 27, 2002Paper

Rhodococcus erythropolis ATCC 25544 as a suitable source of cholesterol oxidase: cell-linked and extracellular enzyme synthesis, purification and concentration

BMC Biotechnology
Mar M SojoF García-Carmona

Abstract

The suitability of the strain Rhodococcus erythropolis ATCC 25544 grown in a two-liter fermentor as a source of cholesterol oxidase has been investigated. The strain produces both cell-linked and extracellular cholesterol oxidase in a high amount, that can be extracted, purified and concentrated by using the detergent Triton X-114. A spray-dry method of preparation of the enzyme inducer cholesterol in Tween 20 was found to be superior in both convenience and enzyme synthesis yield to one of heat-mixing. Both were similar as far as biomass yield is concerned. Cell-linked cholesterol oxidase was extracted with Triton X-114, and this detergent was also used for purification and concentration, following temperature-induced detergent phase separation. Triton X-114 was utilized to purify and to concentrate the cell-linked and the extracellular enzyme. Cholesterol oxidase was found mainly in the resulting detergent-rich phase. When Triton X-114 concentration was set to 6% w/v the extracellular, but not the cell-extracted enzyme, underwent a 3.4-fold activation after the phase separation process. This result is interpreted in the light of interconvertible forms of the enzyme that do not seem to be in equilibrium. Fermentation yielded 3...Continue Reading

Citations

May 24, 2013·World Journal of Microbiology & Biotechnology·Ram NiwasC K M Tripathi
Apr 7, 2021·Biotechnology Advances·Youxiang Liang, Huimin Yu
Jul 21, 2004·The Journal of Steroid Biochemistry and Molecular Biology·V M NikolayevaM V Donova
Jul 16, 2010·Microbiological Research·Laura Fernández de Las HerasOliver Drzyzga

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