Rhodopsin forms a dimer with cytoplasmic helix 8 contacts in native membranes.

Biochemistry
Adam M KneppThomas Huber

Abstract

G protein-coupled receptors form dimers and higher-order oligomers in membranes, but the precise mode of receptor-receptor interaction remains unknown. To probe the intradimeric proximity of helix 8 (H8), we conducted chemical cross-linking of endogenous cysteines in rhodopsin in disk membranes. We identified a Cys316-Cys316 cross-link using partial proteolysis and liquid chromatography with mass spectrometry. These results show that a symmetric dimer interface mediated by H1 and H8 contacts is present in native membranes.

References

Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·G F Schertler, P A Hargrave
Jan 10, 2003·Nature·Dimitrios FotiadisKrzysztof Palczewski
Mar 29, 2003·The Journal of Biological Chemistry·Yan LiangAndreas Engel
Aug 27, 2004·The EMBO Journal·Jonathan J RuprechtGebhard F X Schertler
Oct 19, 2004·The Journal of Biological Chemistry·Beata JastrzebskaKrzysztof Palczewski
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·David SalomKrzysztof Palczewski
Mar 4, 2008·Journal of Molecular Biology·Sourabh BanerjeeThomas P Sakmar
Jun 12, 2010·Trends in Biochemical Sciences·Krzysztof Palczewski
Jul 21, 2011·Nature·Søren G F RasmussenBrian K Kobilka

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Citations

Jul 3, 2013·Experimental Brain Research·Katharine Herrick-Davis
Jun 12, 2012·Journal of the American Chemical Society·Xavier PerioleThomas Huber
Aug 24, 2012·PLoS Computational Biology·Jennifer M JohnstonMarta Filizola
Feb 11, 2014·Molecular Biology and Evolution·Ryan K SchottBelinda S W Chang
Oct 9, 2014·Nature Communications·Michael A GorenAnant K Menon
Jun 26, 2014·Human Molecular Genetics·Elvir BecirovicMartin Biel
Feb 12, 2014·Pharmacological Reviews·Sergi FerréXavier Guitart
Dec 1, 2012·Expert Review of Proteomics·Lars Konermann, Yan Pan
Jun 17, 2014·The Journal of Physiology·Nevin A Lambert, Jonathan A Javitch
Nov 7, 2012·Trends in Pharmacological Sciences·Marta Filizola, Lakshmi A Devi
Oct 10, 2013·Scientific Reports·Sayan MondalHarel Weinstein
Oct 24, 2012·Biochimica Et Biophysica Acta·Kathryn L Chapman, John B C Findlay
Dec 17, 2014·Nature Chemical Biology·Li XuePhilippe Rondard
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tao ZhangYingbin Fu
Aug 16, 2016·Frontiers in Neuroscience·Elvir BecirovicStylianos Michalakis
Nov 22, 2016·International Journal of Molecular Sciences·Takaaki SatoHiroyoshi Matsumura
May 24, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tamar GetterKrzysztof Palczewski
Jul 28, 2017·The Journal of Biological Chemistry·Keiji Seno, Fumio Hayashi
Jan 9, 2019·Frontiers in Molecular Neuroscience·Jingjing Zang, Stephan C F Neuhauss
Jul 1, 2020·Frontiers in Molecular Neuroscience·Alexandra Fletcher-JonesKevin A Wilkinson
Mar 15, 2015·Molecular Pharmacology·María José Varela ListeGraeme Milligan
Aug 11, 2019·The Journal of Biological Chemistry·Dorothy Yanling ZhaoKrzysztof Palczewski
Oct 18, 2013·The Journal of Biological Chemistry·Jianxin HuJürgen Wess
Sep 4, 2015·The Journal of Biological Chemistry·Beata JastrzebskaKrzysztof Palczewski
Oct 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anette Kaiser, Irene Coin
Jan 20, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthias KotthoffDietmar Krautwurst
Feb 17, 2021·Pflügers Archiv : European journal of physiology·Paul S-H Park
Feb 17, 2021·Journal of Cell Science·Xiaoming FangJarema J Malicki
Mar 12, 2021·Bioscience, Biotechnology, and Biochemistry·Ying LiuTian-Rui Xu
May 20, 2021·Scientific Reports·George KhelashviliAnant K Menon
Dec 29, 2018·Biochemistry·Chamnongsak Ken ChanthamontriDaisy W Leung
Feb 14, 2019·Chemical Reviews·Valentina CorradiD Peter Tieleman
Aug 21, 2019·Journal of Chemical Information and Modeling·Ismail ErolSerdar Durdagi

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