RhoGDI-3 is a new GDP dissociation inhibitor (GDI). Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG.

The Journal of Biological Chemistry
G ZalcmanBirgitta Olofsson

Abstract

RhoB is a small GTP-binding protein highly homologous to the RhoA protein. While RhoA is known to regulate the assembly of focal adhesions and stress fibers in response to growth factors, the function of RhoB remains unknown. We have reported that the transient expression of the endogenous RhoB protein is regulated during the cell cycle, contrasting with the permanent RhoA protein expression (). Using the yeast two-hybrid system to characterize proteins interacting with RhoB, we identified a new mouse Rho GDP dissociation inhibitor, referenced as RhoGDI-3. The NH2-terminal alpha helix of RhoGDI-3 is strongly amphipatic and differs thus from that found in previously described bovine, human, and yeast RhoGDI proteins and mouse and human D4/Ly-GDIs. Contrary to the cytosolic localization of all known GDI proteins, acting on Rab or Rho, RhoGDI-3 is associated to a Triton X-100-insoluble membranous or cytoskeletal subcellular fraction. In the two-hybrid system, RhoGDI-3 interacts specifically with GDP- and GTP-bound forms of post-translationally processed RhoB and RhoG proteins, both of which show a growth-regulated expression in mammalian cells. No interaction is found with RhoA, RhoC, or Rac1 proteins. We show that GDI-3 is able t...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·P AdamsonA Hall
Sep 10, 1992·Nature·J SettlemanR A Weinberg
Jul 1, 1992·Molecular and Cellular Biology·S VincentP Fort
Feb 1, 1991·Human Genetics·M F Rousseau-MerckR Berger
Apr 1, 1990·Molecular and Cellular Biology·G T DrivasP D'Eustachio
Dec 1, 1988·Microbiological Reviews·I Herskowitz
Mar 25, 1988·Nucleic Acids Research·P ChardinA Tavitian
May 1, 1985·Cell·P Madaule, R Axel
Sep 22, 1995·The Journal of Biological Chemistry·V Jullien-FloresJ H Camonis
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J V PlatkoB Lim
Jun 16, 1995·Cell·M P PeppelenboschJ L Bos
Oct 1, 1994·Protein Science : a Publication of the Protein Society·J W SealeG D Rose
Nov 30, 1994·Biochemical and Biophysical Research Communications·N BourmeysterP V Vignais
Dec 16, 1993·Nature·M S Boguski, F McCormick
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P ScherleL M Staudt
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M LeliasB Lim
Jan 5, 1996·The Journal of Biological Chemistry·O DorseuilG Gacon
Apr 26, 1996·The Journal of Biological Chemistry·T K Nomanbhoy, R Cerione

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May 18, 1999·International Journal of Cancer. Journal International Du Cancer·G FritzB Kaina
Feb 19, 2000·Biochemical and Biophysical Research Communications·G Fritz, B Kaina
Jun 22, 2012·Cancer Metastasis Reviews·Erin M Griner, Dan Theodorescu
Jun 1, 2005·Trends in Cell Biology·Céline DerMardirossian, Gary M Bokoch
Jul 23, 2011·Nature Reviews. Molecular Cell Biology·Rafael Garcia-MataKeith Burridge
Jul 20, 1999·European Journal of Biochemistry·J FauréM C Dagher
Dec 3, 1999·European Journal of Biochemistry·S F YapL Lim
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Dec 13, 2012·International Journal of Oncology·Yong-Sheng JiangTakahide Ota
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·C N AdraB Lim
Jul 26, 2011·Molecular Membrane Biology·Sandra CitiPamela Pulimeno
Dec 29, 1998·Cell Adhesion and Communication·P F Lebowitz, G C Prendergast
Sep 30, 2005·Traffic·Estelle DransartJacqueline Cherfils
May 19, 2012·Cytoskeleton·Christian Gonzalez-BillaultAlfredo Caceres
Sep 3, 2013·Journal of Neuroscience Research·Jiao WangTieqiao Wen
Mar 4, 2014·International Journal of Cancer. Journal International Du Cancer·Hee Jun ChoJiyun Yoo
Dec 9, 2014·Small GTPases·Antje SchaeferPeter L Hordijk
Mar 10, 2015·The Biochemical Journal·Robert GoggsAlastair W Poole
Feb 27, 2016·Journal of Cellular Physiology·Mamoru FujiwaraMasaaki Tatsuka
Oct 27, 2004·Experimental Cell Research·Ann P Wheeler, Anne J Ridley
Nov 23, 2016·Small GTPases·Francisco M Vega, Anne J Ridley
Sep 22, 2009·Cellular Signalling·Heike BielekCeline Dermardirossian
Nov 19, 2003·Journal of Neurochemistry·E M O'KaneB J Morris

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