RhoGDIβ Inhibits Bone Morphogenetic Protein 4 (BMP4)-induced Adipocyte Lineage Commitment and Favors Smooth Muscle-like Cell Differentiation.

The Journal of Biological Chemistry
Hai-Yan HuangQi-Qun Tang

Abstract

The integration of signals involved in deciding the fate of mesenchymal stem cells is largely unknown. We used proteomics profiling to identify RhoGDIβ, an inhibitor of the small G-protein Rho family, as a component that regulates commitment of C3H10T1/2 mesenchymal stem cells to the adipocyte or smooth muscle cell lineage in response to bone morphogenetic protein 4 (BMP4). RhoGDIβ is notably down-regulated during BMP4-induced adipocytic lineage commitment of C3H10T1/2 mesenchymal stem cells, and this involves the cytoskeleton-associated protein lysyl oxidase. Excess RhoGDIβ completely prevents BMP4-induced commitment to the adipocyte lineage and simultaneously stimulates smooth muscle cell commitment by suppressing the activation of Rac1. Overexpression of RhoGDIβ induces stress fibers of F-actin by a process involving phosphomyosin light chain, indicating that cytoskeletal tension regulated by RhoGDIβ contributes to determining adipocyte versus myocyte commitment. Furthermore, the overexpression of RacV12 (constitutively active form of Rac1) totally rescues the inhibition of adipocyte commitment by RhoGDIβ, simultaneously preventing formation of the smooth muscle-like phenotype and disrupting the stress fibers in cells overex...Continue Reading

References

Dec 1, 1978·Metabolism: Clinical and Experimental·P R JohnsonJ Hirsch
Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Sep 1, 1974·The British Journal of Nutrition·J TremolieresB Plumas
Sep 29, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Feb 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H E YoungP A Lucas
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·R F PereiraD J Prockop
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Jun 3, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dan TheodorescuH F Frierson
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Qi-Qun TangM Daniel Lane
Aug 9, 2005·The Biochemical Journal·Athanassios Dovas, John R Couchman
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Aron B Jaffe, Alan Hall
Jan 28, 2006·Journal of Cellular Biochemistry·Stacey L PayneDawn A Kirschmann
Mar 11, 2008·Expert Review of Cardiovascular Therapy·Harold E BaysRobert R Henry
Oct 7, 2008·Cell·Matthew S RodehefferJeffrey M Friedman
Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Haiyan HuangQi-Qun Tang
Nov 28, 2012·Biochemical Society Transactions·Alan Hall
Jun 12, 2013·The International Journal of Biochemistry & Cell Biology·Shan-Shan WangQi-Qun Tang
Apr 9, 2014·Cell Metabolism·Jonathan Z LongBruce M Spiegelman

❮ Previous
Next ❯

Citations

Jul 23, 2015·Aging Clinical and Experimental Research·Umberto TarantinoElena Bonanno
Jul 3, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yangfan NieXianghua Yan
May 20, 2020·Calcified Tissue International·Marie-Eva Pickering, Roland Chapurlat
Jun 30, 2019·Journal of Muscle Research and Cell Motility·TecLino AfewerkiDerek Warren

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.