RHOXF2 gene, a new candidate gene for spermatogenesis failure

Basic and Clinical Andrology
Christophe FrainaisFrançois Vialard

Abstract

Genes involved in testicular differentiation, spermatogenesis, proliferation and apoptosis of germ cells have been shown to evolve rapidly and display rapid DNA changes. These genes are therefore good candidates for explaining impairments in spermatogenesis. Initial studies of some of these genes appear to confirm this hypothesis. The RHOXF2 candidate gene belongs to the RHOX family clustered in Xq24 and is specifically expressed in the testis. It contains four exons and codes for a 288 amino acid (aa) transcription factor. It has a high degree of homology (>99.9%) with its paralogue RHOXF2B, which is also preferentially expressed in the testis. To sequence RHOXF2 and RHOXF2B in intracytoplasmic sperm injection (ICSI) patients and identify any single-nucleotide polymorphisms (SNPs) associated with impaired spermatogenesis. A cohort of 327 patients in ICSI programmes at Poissy and Bichat hospitals. All patients gave their written, informed consent to participation. One hundred patients had unaffected spermatogenesis and 227 displayed impaired spermatogenesis. The four exons in each of RHOXF2 and RHOXF2B were sequenced in 47 patients with oligospermia or non-obstructive azoospermia. Given that exons 2 and 3 were found to harbour ...Continue Reading

References

Dec 1, 1996·Human Genetics·L StuppiaG Palka
Jul 1, 1997·Mechanisms of Development·W W BranfordS S Potter
Aug 1, 1997·Molecular Human Reproduction·A YoshidaM Shirai
May 1, 2002·The Biochemical Journal·Christoph GeserickBernard Haendler
Feb 6, 2003·Molecular Human Reproduction·Hiromitsu TanakaYoshitake Nishimune
Jul 9, 2004·Genetics·Xiaoxia Wang, Jianzhi Zhang
Nov 25, 2004·European Journal of Human Genetics : EJHG·Katrien StouffsInge Liebaers
Feb 15, 2005·Cell·James A MacleanMiles F Wilkinson
May 5, 2005·PLoS Biology·Rasmus NielsenMichele Cargill
Jun 23, 2005·Reproductive Biomedicine Online·Darius A PaduchPeter N Schlegel
Sep 20, 2005·Fertility and Sterility·Greg L ChristensenDouglas T Carrell
Oct 4, 2005·Journal of Medical Genetics·N IguchiN B Hecht
Feb 24, 2006·PLoS Biology·Benjamin F VoightJonathan K Pritchard
Feb 24, 2006·Genesis : the Journal of Genetics and Development·James A MacLeanMiles F Wilkinson
Apr 4, 2006·Human Reproduction Update·Rafael Oliva
May 10, 2006·PLoS Computational Biology·Leonardo ArbizaHernán Dopazo
Aug 24, 2006·Fertility and Sterility·Terumi MoriTeruaki Iwamoto
Jun 26, 2007·Human Molecular Genetics·Xiaoxia Wang, Jianzhi Zhang

❮ Previous
Next ❯

Citations

Apr 22, 2020·Andrologia·Sezgin Gunes, Sandro C Esteves
Jun 15, 2017·European Journal of Endocrinology·Albrecht Röpke, Frank Tüttelmann
Jun 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Kun TanMiles F Wilkinson

❮ Previous
Next ❯

Methods Mentioned

BETA
PCRs
PCR

Software Mentioned

Oligo
Seqscape®
Statview

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis