PMID: 9438878Aug 1, 1997Paper

Rhythmic expression of a PER-reporter in the Malpighian tubules of decapitated Drosophila: evidence for a brain-independent circadian clock

Journal of Biological Rhythms
D M HegeJ M Giebultowicz

Abstract

The protein product (PER) of the Drosophila clock gene, period (per), is involved in a molecular feedback loop in which PER inhibits the transcription of its own mRNA. This feedback causes the PER protein to cycle in a circadian manner, and this cycling in specific regions of the brain (the presumed location of the central pacemaker) is responsible for the rhythmicity of locomotor activity and possibly eclosion. PER has also been detected in several nonneural tissues in the abdomen, but whether PER exhibits free-running and light-sensitive cycles in any of these tissues is not known. In this study, the authors assayed the spatial and temporal distribution of a PER-reporter expressed in transgenic flies carrying a per-lacZ construct, which was shown to cycle in per-expressing brain cells. The authors demonstrate that this PER-reporter fusion protein cycles in the Malpighian tubules, showing first cytoplasmic accumulation, which is then followed by translocation of the signal into the nucleus. To test whether this rhythm was controlled by the brain, flies were decapitated and assayed for 3 days after decapitation. Expression patterns of PER-reporter in decapitated flies were nearly identical to those in intact flies reared in nor...Continue Reading

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Citations

Feb 14, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kenji TomiokaTaishi Yoshii
May 13, 2011·Cell and Tissue Research·Kerstin I Mehnert, Rafael Cantera
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