PMID: 1063391Apr 1, 1976

Ribonuclease H of calf thymus: substrate specificity, activation, inhibition

Proceedings of the National Academy of Sciences of the United States of America
J G StavrianopoulosE Chargaff

Abstract

When the action of highly purified specimens of ribonuclease H (hybrid nuclease; RNA-DNA hybrid ribonucleotidohydrolase; EC 3.1.4.34) of calf thymus on a wide selection of homopolymer hybrids was studied, the extent, and even the occurrence, of hydrolysis was found to be governed by the interplay of several factors: the composition of the ribo strand, the length of the deoxyribo strand, and the nature of the activating metal. Mn2+ activates the enzymic cleavage of all hybrid combinations, Mg2+ only of those containing purine ribo strands, Co2+ only of poly(A) hybrids. A 1:1 hybrid of phage f1 DNA and RNA is, however, split in the presence of any of these activators. Hybrids with deoxyribo tetranucleotides can still be cleaved, but not with dinucleotides. The behavior of hybrids containing covalently linked runs of ribo and deoxyribopolynucleotides was studied with the hybrid poly(dT)-poly(A)7-(dA)X]. This hybrid is attacked by ribonuclease H so that the bulk of the resulting poly(dA) still retains one covalently linked riboadenylic acid end group, whereas a small proportion carries a ribo dinucleotide. Inhibition studies showed that ribonuclease H is inactivated irreversibly by pretreatment with S-adenosylmethionine at 35 degre...Continue Reading

References

Jun 1, 2001·Journal of Biochemical and Biophysical Methods·E ZamaratskiJ Chattopadhyaya
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J G Starvianopoulos, E Chargaff
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Stavrianopoulos, E Chargaff
Sep 11, 1979·Nucleic Acids Research·H Donis-Keller
Aug 1, 1981·Environmental Health Perspectives·K W Jennette
Sep 25, 2007·Biochemical and Biophysical Research Communications·Avvaru N Suhasini, Ravi Sirdeshmukh
Mar 15, 1977·European Journal of Biochemistry·W BüsenP Hausen

Citations

Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J P LeisJ Hurwitz
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·W Keller
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J G Stavrianopoulos, E Chargaff
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J G StavrianopoulosE Chargaff
Nov 1, 1967·European Journal of Biochemistry·F E Farber, E Chargaff
Jun 1, 1970·European Journal of Biochemistry·P Hausen, H Stein
Dec 1, 1957·Biochimica Et Biophysica Acta·G FELSENFELD, A Rich
Aug 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·A Rich

Related Concepts

Poly T
Structure of Calf of Leg
Cations, Divalent
S-Adenosylhomocysteine
Adenine Polynucleotides
Structure-Activity Relationship
Neoplasm of Uncertain or Unknown Behavior of Thymus
DNA, Viral
Cattle calf (organism)
Bos taurus

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