Nov 1, 1981

Ribonuclease of Entamoeba invadens

Molecular and Biochemical Parasitology
D L WellerC Specht

Abstract

Two bands of RNAase activity were observed on gel electrophoresis of fractions obtained during partial purification of the RNAase of Entamoeba invadens. The faster migrating band which was responsible for most of the RNAase activity was isolated by gel electrophoresis. A pI of 5.5 was determined for this RNAase activity and estimates of its molecular weight and sedimentation coefficient gave values of about 25 000 and approximately 2.8 S. It exhibited maximum activity around pH 6, and digestion of RNA showed a pattern expected of an endoribonuclease.

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Citations

Mentioned in this Paper

Centrifugation, Density Gradient
Cations, Divalent
Entamoeba
Alkaline Ribonuclease
Substrate Specificity
Polyribonucleotides
Hydrogen-Ion Concentration
Isoelectric Point
RNA

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