PMID: 4901707Oct 1, 1969Paper

Ribonuclease V of escherichia coli. I. Dependence on ribosomes and translocation

Proceedings of the National Academy of Sciences of the United States of America
M KuwanoD Schlessinger

Abstract

A new RNase activity, tentatively named RNase V, was found in cell-free extracts of E. coli. This activity requires ribosomes, G and T factors, tRNA, K(+) or NH(4) (+), Mg(2+), GTP, and a sulfhydryl compound to degrade poly U, poly A, T4 phage mRNA, or E. coli mRNA. RNase V is specific for mRNA; it does not attack ribosomal RNA. It is inhibited by antibiotics that decrease breakdown of mRNA in vivo, such as chloramphenicol and streptomycin, and by such agents as 5'-beta, gamma-methylene-guanosine triphosphate, and fusidic acid, which inhibit ribosome-dependent GTPase and translocation of ribosomes along mRNA. The evidence suggests that RNase V is either an integral part of the ribosome or is tightly associated with it, and that it selectively degrades mRNA in intact cells.

References

Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·Y Nishizuka, F Lipmann
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J Lucas-Lenard, F Lipmann
Aug 21, 1968·Biochemical and Biophysical Research Communications·J J Castles, M F Singer
Jan 1, 1967·Biochimica Et Biophysica Acta·G HartmannJ Nüesch
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R ErtelH Weissbach
Mar 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·P F Spahr, R F Gesteland
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J Gordon
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D E MorseC Yanofsky
Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·G P Tocchini-Valentini, E Mattoccia
Dec 9, 1968·Biochemical and Biophysical Research Communications·T KinoshitaN Tanaka
Jun 14, 1969·Journal of Molecular Biology·L LuzzattoD Schlessinger
Feb 14, 1969·Journal of Molecular Biology·R W ErbeP Leder
Feb 28, 1969·Journal of Molecular Biology·J J Castles, M F Singer
Mar 1, 1966·Journal of Molecular Biology·R F Gesteland
Jun 1, 1966·Journal of Molecular Biology·J W Hershey, R E Monro
Jul 28, 1968·Journal of Molecular Biology·B E MadenR E Monro
Apr 1, 1956·Biochimica Et Biophysica Acta·M GRUNBERG-MANAGOS OCHOA
Apr 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·S A YANKOFSKY, S SPIEGELMAN
Mar 23, 1964·Biochimica Et Biophysica Acta·D SCHLESSINGER
Jun 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·T W CONWAY
Dec 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·T W CONWAY, F LIPMANN
Sep 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·C LEVINTHALA HIGA
Oct 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·J H MATTHAEI, M W NIRENBERG
Nov 30, 1967·Biochemical and Biophysical Research Communications·A Stevens, S K Niyogi

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Citations

May 28, 1973·Molecular & General Genetics : MGG·D Apirion
Oct 16, 1973·Molecular & General Genetics : MGG·P KindlerP H Hofschneider
May 20, 1977·Molecular & General Genetics : MGG·M Ono, M Kuwano
May 11, 1973·Molecular and Cellular Biochemistry·S Altman, H D Robertson
Aug 25, 1972·Biochimica Et Biophysica Acta·D Gavard, G de Lamirande
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·M Kuwano, D Schlessinger
Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·W L McLellan, H J Vogel
Jan 1, 1977·Nucleic Acids Research·R Kominami, M Muramatsu
Jun 23, 1973·Biochimica Et Biophysica Acta·H Ceri, P Y Maeba
Feb 5, 1971·Biochemical and Biophysical Research Communications·M Kuwano, D Schlessinger
Aug 28, 1972·Journal of Molecular Biology·M YamamotoM Kuwano
Sep 28, 1971·Journal of Molecular Biology·G MangiarottiM Kuwano
Jun 4, 1971·Biochemical and Biophysical Research Communications·J Eley, K Crawford
Nov 25, 1970·Biochemical and Biophysical Research Communications·A Hirashima, A Kaji
Aug 20, 1971·Biochemical and Biophysical Research Communications·R K Holmes, M F Singer
Apr 4, 1970·Nature·D E Morse, P Primakoff
Oct 18, 1973·European Journal of Biochemistry·G Mangiarotti, E Turco
Jun 16, 1971·Nature: New Biology·M KuwanoD E Morse
Apr 14, 1971·Nature: New Biology·W C Summers
Sep 27, 1973·Molecular & General Genetics : MGG·L Legault-DemareF Gros
Sep 1, 1974·Journal of Bacteriology·G G Khachatourians, D J Tipper

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