Ribonucleic acid synthesis during fruiting body formation in Myxococcus xanthus.

Journal of Bacteriology
B A Smith, M Dworkin

Abstract

A method has been devised that allowed us, for the first time, to pulse-label M. xanthus cells with precursors for ribonucleic acid biosynthesis while they were undergoing fruiting body formation. Using this method, we examined patterns of ribonucleic acid (RNA) accumulation throughout the process of fruiting body formation. As development proceeded, the rate of RNA accumulation increased at two periods of the developmental cycle: once just before aggregation and once late in the cycle, when sporulation was essentially completed. In contrast to vegetatively growing cells, in which only stable RNA species are labeled during a 30-min pulse, the majority of radioactivity found in RNA from 30-min pulse-labeled developing cells was found in an unstable heterodisperse fraction that migrated to the 5S to 16S region of sucrose density gradients and sodium dodecyl sulfate-polyacrylamide gels. This pattern of incorporation could not be induced (i) by a shift down of vegetatively growing cells to a nutritionally poor medium, in which the generation time was increased to that of developing cells during the growth phase, or (ii) by plating of vegetative cells onto the same solid-surface environment as that of developing cells, but which sur...Continue Reading

References

Jan 1, 1979·Annual Review of Microbiology·D KaiserM Dworkin
Oct 1, 1979·Journal of Bacteriology·J Wireman
Jan 1, 1975·Annual Review of Microbiology·M Cashel
Feb 1, 1977·Journal of Bacteriology·J W Wireman, M Dworkin
Aug 15, 1975·Science·J W Wireman, M Dworkin
Feb 1, 1970·Journal of Bacteriology·W S Ramsey, M Dworkin
Apr 1, 1971·Analytical Biochemistry·C P Emerson, T Humphreys
Jun 1, 1968·Journal of Bacteriology·D WhiteD J Tipper
Jun 1, 1969·Journal of Bacteriology·S Z Sudo, M Dworkin
Apr 1, 1966·Journal of Bacteriology·M Dworkin, W Sadler
Dec 1, 1967·Journal of Bacteriology·K Bacon, E Rosenberg
Jun 1, 1980·Journal of Bacteriology·B A Smith, M Dworkin
Aug 1, 1962·Journal of Bacteriology·M DWORKIN

❮ Previous
Next ❯

Citations

Jan 1, 1987·Critical Reviews in Microbiology·L J Shimkets
Nov 1, 1985·Journal of Bacteriology·S M Panasenko
Oct 1, 1992·Current Opinion in Genetics & Development·M A Strauch, J A Hoch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.