PMID: 6164385Mar 3, 1981Paper

Ribonucleic acid synthesis termination protein rho function: effects of conditions that destabilize ribonucleic acid secondary structure

Biochemistry
J P Richardson, M R Macy

Abstract

The dependence fo rate of adenosine 5'-triphosphate (ATP) hydrolysis catalyzed by ribonucleic acid (RNA) synthesis termination protein rho from Escherichia coli with T7 RNA as cofactor is used to probe the nature of the interaction between rho and RNA. In general, reaction conditions that destabilize the secondary structure of the RNA enhance its cofactor activity. This is indicated by the effects of MgCl2 concentration, spermidine, temperature, dimethyl sulfoxide, and pretreatment of the RNA with formaldehyde. These results suggest that a functional interaction between rho and RNA depends either on the presence of a sufficiently large single-stranded region in the RNA or on the ability of rho to unwind double helices in the RNA. It is also shown that changes in reaction conditions that increase RNA secondary structure and decrease the rho protein adenosine triphosphate phosphohydrolase (rhoATPase) activity with isolated T7 RNA also decrease the stringency of rho action in RNA synthesis termination. On the other hand, monovalent salts decrease rhoATPase activity with isolated T7 RNA and binding of rho to T7 RNA independently of the MgCl2 concentration and thus the relative stability of the RNA secondary structure.

Citations

Aug 27, 1981·Biochimica Et Biophysica Acta·S L Bektesh, J P Richardson
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R B Kent, S K Guterman
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·C M Burns, J P Richardson
Jun 11, 1983·Nucleic Acids Research·J L Pinkham, T Platt
May 24, 1985·Nucleic Acids Research·W D MorganP H von Hippel
Jun 5, 1986·Journal of Molecular Biology·J P Richardson, E C Ruteshouser
Jul 5, 1989·Journal of Molecular Biology·E C Ruteshouser, J P Richardson
Aug 8, 2006·Archives of Biochemistry and Biophysics·Tarita BiverMarcella Venturini
Mar 23, 2015·FEMS Microbiology Reviews·Jennifer Shepherd, Michael Ibba
Apr 6, 1990·Biochimica Et Biophysica Acta·J P Richardson

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