Ribose mediated crosslinking of collagen-hydroxyapatite hybrid scaffolds for bone tissue regeneration using biomimetic strategies

Materials Science & Engineering. C, Materials for Biological Applications
Gopal Shankar KrishnakumarMonica Sandri

Abstract

This study explores for the first time the application of ribose as a highly biocompatible agent for the crosslinking of hybrid mineralized constructs, obtained by bio-inspired mineralization of self-assembling Type I collagen matrix with magnesium-doped-hydroxyapatite nanophase, towards a biomimetic mineralized 3D scaffolds (MgHA/Coll) with excellent compositional and structural mimicry of bone tissue. To this aim, two different crosslinking mechanisms in terms of pre-ribose glycation (before freeze drying) and post-ribose glycation (after freeze drying) were investigated. The obtained results explicate that with controlled freeze-drying, highly anisotropic porous structures with opportune macro-micro porosity are obtained. The physical-chemical features of the scaffolds characterized by XRD, FTIR, ICP and TGA demonstrated structural mimicry analogous to the native bone. The influence of ribose greatly assisted in decreasing solubility and increased enzymatic resistivity of the scaffolds. In addition, enhanced mechanical behaviour in response to compressive forces was achieved. Preliminary cell culture experiments reported good cytocompatibility with extensive cell adhesion, proliferation and colonization. Overall, scaffolds d...Continue Reading

Citations

Oct 6, 2018·Journal of Biomedical Materials Research. Part a·Dhivyaa AnandanAmit Kumar Jaiswal
Sep 10, 2020·International Journal of Molecular Sciences·Gangadhar LekshmiWanxi Peng
Mar 12, 2020·Materials·Liane SchusterDorothea Alexander
Aug 11, 2020·Frontiers in Bioengineering and Biotechnology·Alessandra DellaquilaMonica Sandri
Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Elisabetta CampodoniMonica Sandri
Oct 20, 2020·Macromolecular Bioscience·Jia Min LeeWai Yee Yeong
Nov 4, 2020·Journal of Functional Biomaterials·Nunzia GalloLuca Salvatore
Jun 4, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Luca SalvatoreAlessandro Sannino
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Shalini MuthusamyGopal Shankar Krishnakumar

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