Ribosomal 5 S rRNA maturation in Saccharomyces cerevisiae.

The Journal of Biological Chemistry
Y Lee, R N Nazar

Abstract

The maturation of the ribosomal 5 S RNA in Saccharomyces cerevisiae is examined based on the expression of mutant 5 S rRNA genes, in vivo, and a parallel analysis of RNA processing, in vitro. Both types of analysis indicate that 5 S rRNA processing is not dependent on the nucleotide sequence of either the external transcribed spacer or the mature 5 S rRNA. The results further indicate the RNA is processed by an exonuclease activity which is limited primarily or entirely by helix I, the secondary structure formed between the mature and interacting termini. The 5 S RNA binding protein (YL3) also appears not to influence directly the maturation process, but rather to play a role in protecting the rRNA from further degradation by "housekeeping" nucleases. Taken together, the results continue to support a "quality control" function which helps to ensure that during maturation only normal precursors are processed and assembled into active ribosomes.

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Citations

Nov 20, 2001·Molecular and Cellular Biology·M W SmithJ D Dinman
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z LiM P Deutscher
Jun 5, 2008·The Journal of Biological Chemistry·Sadeq NabaviRoss N Nazar
Dec 10, 2002·The Journal of Biological Chemistry·Yoon Lee, Ross N Nazar
Nov 24, 1999·Molecular and Cellular Biology·D KresslerJ de La Cruz
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Martin Ciganda, Noreen Williams
Oct 21, 2014·Wiley Interdisciplinary Reviews. RNA·Antonio Fernández-PevidaJesús de la Cruz
Mar 7, 2008·The Journal of Biological Chemistry·Sadeq Nabavi, Ross N Nazar

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