Ribosomal frameshifting and dual-target antiactivation restrict quorum-sensing-activated transfer of a mobile genetic element

Proceedings of the National Academy of Sciences of the United States of America
Joshua P RamsayC W Ronson

Abstract

Symbiosis islands are integrative and conjugative mobile genetic elements that convert nonsymbiotic rhizobia into nitrogen-fixing symbionts of leguminous plants. Excision of the Mesorhizobium loti symbiosis island ICEMlSym(R7A) is indirectly activated by quorum sensing through TraR-dependent activation of the excisionase gene rdfS. Here we show that a +1 programmed ribosomal frameshift (PRF) fuses the coding sequences of two TraR-activated genes, msi172 and msi171, producing an activator of rdfS expression named Frameshifted excision activator (FseA). Mass-spectrometry and mutational analyses indicated that the PRF occurred through +1 slippage of the tRNA(phe) from UUU to UUC within a conserved msi172-encoded motif. FseA activated rdfS expression in the absence of ICEMlSym(R7A), suggesting that it directly activated rdfS transcription, despite being unrelated to any characterized DNA-binding proteins. Bacterial two-hybrid and gene-reporter assays demonstrated that FseA was also bound and inhibited by the ICEMlSym(R7A)-encoded quorum-sensing antiactivator QseM. Thus, activation of ICEMlSym(R7A) excision is counteracted by TraR antiactivation, ribosomal frameshifting, and FseA antiactivation. This robust suppression likely dampen...Continue Reading

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Citations

Nov 28, 2015·Trends in Microbiology·Philippe RemigiCatherine Masson-Boivin
Mar 5, 2016·Mobile Genetic Elements·Joshua P Ramsay, Clive W Ronson
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Timothy L HaskettJoshua P Ramsay
Jan 31, 2018·Nature Reviews. Microbiology·Philip PooleJason Terpolilli
Mar 27, 2020·Genes·Ginaini Grazielli Doin de MouraDelphine Capela
Nov 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jun LingJun Zhu
Apr 4, 2017·FEMS Microbiology Reviews·François DelavatJan Roelof van der Meer
Feb 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Svenja B KroegerJulien G A Martin
Jul 12, 2020·Applied and Environmental Microbiology·Shaun FergusonClive W Ronson

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