PMID: 11607297Jun 15, 1992Paper

Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures

Proceedings of the National Academy of Sciences of the United States of America
A ConconiC A Ryan

Abstract

The accessibility of regulatory molecules to specific DNA sequences and chromatin regions in the nucleus is crucial to gene expression. In this study, we examined the chromatin structure in tomato leaf cells and in exponentially growing tomato cell suspension cultures. The structure of ribosomal chromatin was investigated by micrococcal nuclease and psoralen photocrosslinking. We showed that ribosomal genes in tomato are folded into two distinct types of chromatin: an open chromatin conformation and a closed nucleosomecontaining chromatin. In contrast to previous findings in Friend cells, where half of the ribosomal genes were found to be complexed within an inactive chromatin structure, we demonstrated that the canonical nucleosome-containing chromatin is present in the majority (approximately 80%) of the tomato rRNA-encoding DNA clusters. The minor open chromatin population (approximately 20% of the ribosomal genes) could be detected only after analysis following psoralen crosslinking. The relative amounts of the two ribosomal chromatin structures are similar in stationary and exponentially growing cells. This suggests that the proportions of open and closed chromatin structures present in either stationary or exponentially g...Continue Reading

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Citations

May 10, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Cristina SanzJosé Luís Díez
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·M C Risueño, P S Testillano
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Sep 12, 2003·DNA Repair·Vasilissa I Manova, Lubomir M Stoilov
Apr 29, 1998·Nucleic Acids Research·R E Wellinger, J M Sogo
Aug 12, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Martin ToussaintAntonio Conconi
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Maxime TremblayAntonio Conconi
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Antonio ConconiMichael J Smerdon
Oct 16, 2012·Biochimica Et Biophysica Acta·Ingrid Grummt, Gernot Längst
Mar 11, 2016·Protist·Juan C Vizuet-de-RuedaSantiago Martínez-Calvillo
Aug 26, 2006·Mutation Research·Vasilissa ManovaLubomir Stoilov
Oct 23, 2014·Journal of Cellular Physiology·Le Xuan Truong NguyenBeverly S Mitchell
Mar 29, 2018·Annual Review of Biochemistry·Samim Sharifi, Holger Bierhoff
Nov 2, 2005·Molecular and Cellular Biology·Antonio ConconiMichael J Smerdon
Sep 27, 2006·Molecular and Cellular Biology·Junqiang Ye, Thomas H Eickbush
May 16, 2003·Molecular and Cellular Biology·Danna G Eickbush, Thomas H Eickbush
Jun 25, 2015·Microbiology Spectrum·Thomas H Eickbush, Danna G Eickbush

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