Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus
Abstract
A particulate fraction has been isolated from detergent-prepared HeLa cell nuclei. The fraction consists largely of organelles that resemble the nucleoli of intact cells. The 45S RNA that is precursor to 28S and 18S ribosomal RNA is associated with the fraction. The 32S RNA that is labeled after the 45S RNA and is the apparent precursor to 28S RNA is also associated with the fraction. The nucleoplasm contains 28S RNA that behaves as an intermediate between the 32S nucleolar RNA and the 28S cytoplasmic RNA.
Citations
Ribosome-like granules within areas of the perinuclear space in cells of 13--14 somite chick embryos
The use of deoxyribonuclease in the isolation of nucleoli and purification of nuclear RNA--a caution
In vivo interaction of nucleophosmin/B23 and protein C23 during cell cycle progression in HeLa cells
Rapid isolation of nucleoli from detergent purified nuclei of various tumor and tissue culture cells
Studies with the schistosomacide hycanthone: inhibition of macromolecular synthesis and its reversal
Nuclear metabolism of ribosomal RNA in growing, methionine-limited, and ethionine-treated HeLa cells
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