Ribosome bypassing elicited by tRNA depletion

Molecular Microbiology
Dale LindsleyGabriel Guarneros

Abstract

Ribosome bypassing refers to the ability of the ribosome::peptidyl-tRNA complex to slide down the message without translation to a site several or dozens of nucleotides downstream and resume protein chain elongation there. The product is an isoform of a protein with a 'coding' gap corresponding to the region of the message which was bypassed. Previous work showed that ribosome bypassing was strongly stimulated at 'hungry' codons calling for a tRNA whose aminoacylation was limited. We have now used the 'minigene' phenomenon to ascertain whether depletion of the pool of specific isoacceptors has a similar effect. High level expression of plasmid-borne minigenes results in the sequestration as peptidyl-tRNA of tRNA cognate to the last triplet of the minigene, thereby limiting protein synthesis for lack of the tRNA in question. We find that induction of a minigene ending in AUA stimulates bypassing at an AUA codon, but not in a control sequence with AGA at the test position; induction of a minigene ending in AGA stimulates bypassing at the latter but not the former. Induction of the AUA minigene also stimulates both leftward and rightward frameshifting at 'shifty' sequences containing an AUA codon. The normal, background frequency ...Continue Reading

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Citations

Oct 29, 2003·Biochimica Et Biophysica Acta·Michael O'Connor
Mar 23, 2004·Gene·Norma-Angélica OviedoGabriel Guarneros
Aug 20, 2004·Nucleic Acids Research·Luis Rogelio Cruz-VeraGabriel Guarneros
Aug 30, 2007·Nucleic Acids Research·Efraín Zamora-RomoGabriel Guarneros
Oct 27, 2012·Journal of Clinical Microbiology·Brenden C SmithCaroline E Cameron
Oct 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonathan GallantDale Lindsley
Mar 5, 2013·Journal of Theoretical Biology·Peter R Wills
Feb 21, 2006·Biochimie·Luis Delgado-OlivaresJavier Hernandez-Sanchez
May 14, 2005·Journal of Molecular Biology·Dale LindsleyAshley Fontelera
Oct 3, 2006·BioFactors·Lijun ZhaoEthan Will Taylor

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