Ribosome display: cell-free protein display technology

Briefings in Functional Genomics & Proteomics
M He, M J Taussig

Abstract

Ribosome display is a cell-free system for the in vitro selection of proteins and peptides from large libraries. It uses the principle of coupling individual nascent proteins (phenotypes) to their corresponding mRNA (genotypes), through the formation of stable protein-ribosome-mRNA (PRM) complexes. This permits the simultaneous isolation of a functional nascent protein, through affinity for a ligand, together with the encoding mRNA, which is then converted and amplified as DNA for further manipulation, including repeated cycles or protein expression. Ribosome display has a number of advantages over cell-based systems such as phage display; in particular, it can display very large libraries without the restriction of bacterial transformation. It is also suitable for generating toxic, proteolytically sensitive and unstable proteins, and allows the incorporation of modified amino acids at defined positions. In combination with polymerase chain reaction (PCR)-based methods, mutations can be introduced efficiently into the selected DNA pool in subsequent cycles, leading to continuous DNA diversification and protein selection (in vitro protein evolution). Both prokaryotic and eukaryotic ribosome display systems have been developed an...Continue Reading

Citations

Dec 21, 2005·Applied Microbiology and Biotechnology·Matthias Paschke
Jun 2, 2011·Briefings in Functional Genomics·Christopher G UllmanR Neil Cooley
Apr 12, 2007·BMC Biotechnology·George KopsidasGreg Coia
Feb 28, 2013·Therapeutic Delivery·Craig RussellAfzal R Mohammed
Jul 8, 2005·Expert Review of Proteomics·Mingyue He, Farid Khan
Feb 16, 2005·Expert Opinion on Biological Therapy·Maria A T Groves, Jane K Osbourn
Jan 27, 2006·Expert Opinion on Biological Therapy·Achim RotheBarbara E Power
Oct 20, 2015·Chembiochem : a European Journal of Chemical Biology·Anne ZemellaStefan Kubick
Aug 18, 2009·Journal of Molecular Biology·Alexander EggelMonique Vogel
Mar 26, 2009·Journal of Microbiological Methods·Liancheng LeiJiaqi Wang
May 12, 2009·Seminars in Cell & Developmental Biology·Paul J ConroyRichard J O'Kennedy
Jul 16, 2008·Biochemical and Biophysical Research Communications·Swey-Shen ChenTeresa J Barankiewicz
Apr 24, 2007·The FEBS Journal·Ykelien L BoersmaWim J Quax
Mar 22, 2005·Journal of Immunological Methods·Mingyue He, Michael J Taussig
Feb 25, 2015·Biotechnology Advances·Mariia DarmostukTomas Ruml
Sep 21, 2013·Drug Discovery Today·Maryam Hamzeh-MivehroudSiavoush Dastmalchi
Jan 29, 2008·Genomics·Matthias Harbers
Jan 17, 2012·Archives of Biochemistry and Biophysics·Noa Harel Inbar, Itai Benhar
Oct 25, 2014·PloS One·Giacomo BastianelliJean-Christophe Barale
Feb 22, 2017·International Journal of Molecular Sciences·Savita Nandal, Tal Burt
Apr 27, 2018·Expert Opinion on Drug Discovery·Dani SetiawanYang Zhang
Feb 18, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thomas LindnerWalter Mier
May 11, 2019·The American Journal of Tropical Medicine and Hygiene·Adinarayana KunamneniRavi Durvasula
Jan 23, 2020·Physical Chemistry Chemical Physics : PCCP·Celia BlancoIrene A Chen
Jan 22, 2009·Molecular BioSystems·Amanda S Rohrbach, Tobin J Dickerson
Jun 3, 2015·Journal of Separation Science·Gérald PerretEgisto Boschetti
Apr 18, 2020·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Congcong ZhangAndrea L J Marschall
Apr 13, 2017·Frontiers in Microbiology·Abdullah F U H SaeedShihua Wang
Apr 28, 2017·Journal of Molecular Microbiology and Biotechnology·Vic NorrisGlenn J White
Jul 2, 2020·Antibodies·Adinarayana KunamneniRavi Durvasula

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