Ribosome recycling factor disassembles the post-termination ribosomal complex independent of the ribosomal translocase activity of elongation factor G

Molecular Microbiology
Toshinobu FujiwaraYoshikazu Nakamura

Abstract

Ribosome recycling factor (RRF) disassembles post-termination ribosomal complexes in concert with elongation factor EF-G freeing the ribosome for a new round of polypeptide synthesis. How RRF interacts with EF-G and disassembles post-termination ribosomes is unknown. RRF is structurally similar to tRNA and is therefore thought to bind to the ribosomal A site and be translocated by EF-G during ribosome disassembly as a mimic of tRNA. However, EF-G variants that remain active in GTP hydrolysis but are defective in tRNA translocation fully activate RRF function in vivo and in vitro. Furthermore, RRF and the GTP form of EF-G do not co-occupy the terminating ribosome in vitro; RRF is ejected by EF-G from the preformed complex. These findings suggest that RRF is not a functional mimic of tRNA and disassembles the post-termination ribosomal complex independently of the translocation activity of EF-G.

References

Mar 27, 1981·Molecular and Cellular Biochemistry·A Parmeggiani, G Sander
Feb 26, 1998·Cell·K S Wilson, H F Noller
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R K AgrawalJ Frank
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·M V RodninaW Wintermeyer
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·D E AgafonovA S Spirin
Jul 13, 2000·Journal of Molecular Biology·A SavelsberghW Wintermeyer
Oct 4, 2000·Nature Structural Biology·K S WilsonY Nakamura
Jan 4, 2003·Nature·Urmila B S RawatJoachim Frank
Feb 11, 2003·Trends in Biochemical Sciences·Yoshikazu Nakamura, Koichi Ito
Jun 25, 2003·Molecular Cell·Andreas SavelsberghWolfgang Wintermeyer
Jul 16, 2003·Cell·Andrey V Zavialov, Måns Ehrenberg
Jul 16, 2003·Cell·Mikel ValleJoachim Frank
Sep 10, 2003·The Journal of Biological Chemistry·Michael C KielAkira Kaji

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Citations

Oct 6, 2005·Nucleic Acids Research·Krisana AsanoHyouta Himeno
Oct 30, 2010·Tree Physiology·Sacha BohlerJean-Francois Hausman
Jan 12, 2007·BMC Genomics·Tõnu MargusTanel Tenson
Mar 28, 2009·RNA·Andreas SavelsberghWolfgang Wintermeyer
Dec 26, 2012·Trends in Biochemical Sciences·Elina Nürenberg, Robert Tampé
Sep 1, 2009·Molecular Cell·Masafumi TsuboiNono Takeuchi
May 17, 2005·Molecular Cell·Frank PeskeWolfgang Wintermeyer
Jun 5, 2016·Cellular and Molecular Life Sciences : CMLS·Zhe Lyu, William B Whitman
Nov 3, 2005·The Biochemical Journal·Peng GuoGuozhong Jing
Dec 25, 2019·Nature Structural & Molecular Biology·Dejian ZhouMatthieu G Gagnon
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Yoshikazu Nakamura, Koichi Ito

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