PMID: 6409101Apr 15, 1983Paper

Ribulose bisphosphate carboxylase/oxygenase in toluene-permeabilized Rhodospirillum rubrum

The Biochemical Journal
I Storrø, B A McFadden

Abstract

Toluene-permeabilized Rhodospirillum rubrum cells were used to study activation of and catalysis by the dual-function enzyme ribulose bisphosphate carboxylase/oxygenase. Incubation with CO2 provided as HCO3-, followed by rapid removal of CO2 at 2 degrees C and subsequent incubation at 30 degrees C before assay, enabled a determination of decay rates of the carboxylase and the oxygenase. Half-times at 30 degrees C with 20 mM-Mg2+ were 10.8 and 3.7 min respectively. Additionally, the concentrations of CO2 required for half-maximal activation were 56 and 72 microM for the oxygenase and the carboxylase respectively. After activation and CO2 removal, inactivation of ribulose bisphosphate oxygenase in the presence of 1 mM- or 20mM-Mn2+ was slower than that with the same concentrations of Co2+ or Mg2+. Only the addition of Mg2+ supported ribulose bisphosphate carboxylase activity, as Mn2+, Co2+ and Ni2+ had no effect. A pH increase after activation in the range 6.8-8.0 decreased the stability of the carboxylase but in the range 7.2-8.0 increased the stability of the oxygenase. With regard to catalysis. Km values for ribulose 1,5-bisphosphate4- were 1.5 and 67 microM for the oxygenase and the carboxylase respectively, and 125 microM fo...Continue Reading

References

Jun 5, 1970·Biochemical and Biophysical Research Communications·A C Rutner
Jan 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D Sankoff
Oct 1, 1973·Biochemical and Biophysical Research Communications·M Nishimura, T Akazawa
Oct 28, 1971·Journal of Molecular Biology·A D McLachlan
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·C R Cantor, T H Jukes

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