Ribulose Monophosphate Shunt Provides Nearly All Biomass and Energy Required for Growth of E. coli

ACS Synthetic Biology
Hai HeArren Bar-Even

Abstract

The ribulose monophosphate (RuMP) cycle is a highly efficient route for the assimilation of reduced one-carbon compounds. Despite considerable research, the RuMP cycle has not been fully implemented in model biotechnological organisms such as Escherichia coli, mainly since the heterologous establishment of the pathway requires addressing multiple challenges: sufficient formaldehyde production, efficient formaldehyde assimilation, and sufficient regeneration of the formaldehyde acceptor, ribulose 5-phosphate. Here, by efficiently producing formaldehyde from sarcosine oxidation and ribulose 5-phosphate from exogenous xylose, we set aside two of these concerns, allowing us to focus on the particular challenge of establishing efficient formaldehyde assimilation via the RuMP shunt, the linear variant of the RuMP cycle. We have generated deletion strains whose growth depends, to different extents, on the activity of the RuMP shunt, thus incrementally increasing the selection pressure for the activity of the synthetic pathway. Our final strain depends on the activity of the RuMP shunt for providing the cell with almost all biomass and energy needs, presenting an absolute coupling between growth and activity of key RuMP cycle component...Continue Reading

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Citations

Feb 16, 2020·Metabolites·Hai HeArren Bar-Even
Jul 14, 2020·Biotechnology and Bioengineering·Sebastian WenkArren Bar-Even
Jul 19, 2020·World Journal of Microbiology & Biotechnology·Philibert Tuyishime, Jean Paul Sinumvayo
Nov 18, 2020·Nature Communications·Ari SatanowskiArren Bar-Even
Aug 23, 2019·Current Opinion in Biotechnology·Maciek R Antoniewicz
Oct 28, 2020·Nature Communications·Philipp KellerJulia A Vorholt
Nov 6, 2020·Nature Communications·Monica I EspinosaThomas C Williams
Aug 15, 2018·Metabolic Engineering·Sammy PontrelliJames C Liao
Sep 3, 2018·Metabolic Engineering·Chang-Ting ChenJames C Liao

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