Rice choline monooxygenase (OsCMO) protein functions in enhancing glycine betaine biosynthesis in transgenic tobacco but does not accumulate in rice (Oryza sativa L. ssp. japonica).

Plant Cell Reports
Di LuoYongsheng Liu

Abstract

Glycine betaine (GB) is a compatible quaternary amine that enables plants to tolerate abiotic stresses, including salt, drought and cold. In plants, GB is synthesized through two-step of successive oxidations from choline, catalyzed by choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH), respectively. Rice is considered as a typical non-GB accumulating species, although the entire genome sequencing revealed rice contains orthologs of both CMO and BADH. Several studies unraveled that rice has a functional BADH gene, but whether rice CMO gene (OsCMO) is functional or a pseudogene remains to be elucidated. In the present study, we report the functional characterization of rice CMO gene. The OsCMO gene was isolated from rice cv. Nipponbare (Oryza sativa L. ssp. japonica) using RT-PCR. Northern blot demonstrated the transcription of OsCMO is enhanced by salt stress. Transgenic tobacco plants overexpressing OsCMO results in increased GB content and elevated tolerance to salt stress. Immunoblotting analysis demonstrates that a functional OsCMO protein with correct size was present in transgenic tobacco but rarely accumulated in wild-type rice plants. Surprisingly, a large amount of truncated proteins derived from OsC...Continue Reading

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Citations

Feb 14, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Showkat Ahmad GanieTapan Kumar Mondal
Feb 26, 2020·Hereditas·Haiji SunXiaoli Ma
Feb 23, 2021·Frontiers in Genetics·Susana González-MoralesAdalberto Benavides-Mendoza
May 29, 2021·Plant Physiology and Biochemistry : PPB·Mushtaq Ahmad MalikIrfan Rashid

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