Rice endosperm is cost-effective for the production of recombinant griffithsin with potent activity against HIV.

Plant Biotechnology Journal
Evangelia VamvakaTeresa Capell

Abstract

Protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (HIV) if it is possible to manufacture the components in large quantities at a cost affordable in HIV-endemic regions such as sub-Saharan Africa. We expressed the antiviral lectin griffithsin (GRFT), which shows potent neutralizing activity against HIV, in the endosperm of transgenic rice plants (Oryza sativa), to determine whether rice can be used to produce inexpensive GRFT as a microbicide ingredient. The yield of (OS) GRFT in the best-performing plants was 223 μg/g dry seed weight. We also established a one-step purification protocol, achieving a recovery of 74% and a purity of 80%, which potentially could be developed into a larger-scale process to facilitate inexpensive downstream processing. (OS) GRFT bound to HIV glycans with similar efficiency to GRFT produced in Escherichia coli. Whole-cell assays using purified (OS) GRFT and infectivity assays using crude extracts of transgenic rice endosperm confirmed that both crude and pure (OS) GRFT showed potent activity against HIV and the crude extracts were not toxic towards human cell lines, suggesting they could be administ...Continue Reading

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Citations

Oct 27, 2016·Viruses·Sabrina Lusvarghi, Carole A Bewley
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Evangelia VamvakaTeresa Capell
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sergio Rosales-MendozaSornkanok Vimolmangkang
Mar 7, 2021·Plants·Md Jahidul Islam ShohagXiaoe Yang
Mar 8, 2021·International Journal of Biological Macromolecules·Yousra A El-MaradnyHeba S Selim
Jul 4, 2020·Journal of Biotechnology·Jianlei PangDaichang Yang
May 1, 2021·Vaccines·Jennifer Schwestka, Eva Stoger

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Datasets Mentioned

BETA
CAA39776

Methods Mentioned

BETA
transgenic
ELISA
electrophoresis
glycosylation
Fluorescence
transmission electron microscopy
Fluorescence microscopy
size exclusion chromatography
electron microscopy
PCR

Software Mentioned

OS GRFT

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