Rice-induced secondary metabolite gene expression in Aspergillus nidulans.

Journal of Industrial Microbiology & Biotechnology
Christopher J LacriolaBernard Weisblum

Abstract

Activation of silent biosynthetic gene clusters in fungi remains a challenge for discovery of new useful natural products. In this work, we identify a group of silent secondary metabolite gene clusters in Aspergillus nidulans that are induced by rice (Oryza sativa). Using reverse phase HPLC purification on extracts of rice, we identified the plant hormone gibberellic acid as one compound present in rice extracts that induced these silent genes. Additionally, select secondary metabolite (SM) genes activated by rice were tested for responses to several plant hormones which produced distinctly different transcriptomic profiles in A. nidulans. These observations support the idea that phytohormones play an important role in regulating fungal SM biosynthesis while additionally serving as a source of natural product chemical libraries to screen for useful compounds.

References

Jan 7, 1998·Protein Expression and Purification·C Lu, H P Erickson
Jan 20, 2006·Molecular Microbiology·Dimitrios I Tsitsigiannis, Nancy P Keller
Oct 13, 2006·Organic & Biomolecular Chemistry·Kirstin Scherlach, Christian Hertweck
Jun 19, 2009·Nature Chemical Biology·Christian Hertweck
Apr 27, 2012·The Biochemical Journal·Peter Hedden, Stephen G Thomas
Sep 25, 2012·Applied and Environmental Microbiology·Jennifer GerkeGerhard H Braus
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Jan 7, 2014·Applied Microbiology and Biotechnology·Eva-Maria NiehausBettina Tudzynski
Mar 31, 2015·ACS Chemical Biology·Jessica C AlbrightNeil L Kelleher
Aug 25, 2015·Frontiers in Plant Science·Tünde PusztahelyiIstván Pócsi
Apr 23, 2016·Cell·Yansheng LiuRuedi Aebersold
Oct 13, 2016·Annual Review of Genetics·Juliane MacheleidtAxel A Brakhage
Apr 5, 2017·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Jul 25, 2017·Advances in Applied Microbiology·Hee-Soo ParkJae-Hyuk Yu
Jan 31, 2018·Analytical Chemistry·Carlos GuijasGary Siuzdak
Mar 20, 2018·Microbiological Research·Sonia Salazar-CerezoRebeca D Martínez-Contreras
Jan 4, 2019·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Jan 29, 2019·Frontiers in Plant Science·Josef VrabkaVeronique Bergougnoux

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Citations

Aug 12, 2021·Journal of Industrial Microbiology & Biotechnology·Takayuki MotoyamaHiroyuki Osada

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Methods Mentioned

BETA
targeted gene modification
chip
Assay
RNA-Seq
PCR
electrophoresis

Software Mentioned

Analyst
Illumina Pipeline
GO mapper
goTermMapper
GO slim mapper ”
RSEM ( RNA - Seq by Expectation Maximization
edgeR
Spliced Transcripts Alignment to a Reference ( STAR )

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