Rice peptide deformylase PDF1B is crucial for development of chloroplasts

Plant & Cell Physiology
Sunok MoonGynheung An

Abstract

Because protein synthesis begins with N-formylmethionine in plant endosymbiotic organelles, removal of the formyl group by peptide deformylase (PDF) is essential to allowing the excision of the first methionine. Rice contains three copies (OsPDF1A, OsPDF1B and OsPDF1B2) of the PDF genes. Unlike OsPDF1A and OsPDF1B, OsPDF1B2 is apparently non-functional, with several deleterious substitutions and deletions. OsPDF1A is more strongly expressed in the roots, while OsPDF1B is expressed at higher levels in mature leaves. Transient expression of PDF-green fluorescent protein (GFP) fusion proteins in the protoplasts demonstrates that, unlike OsPDF1A, OsPDF1B is localized in both the chloroplasts and the mitochondria. We used T-DNA insertional alleles to elucidate functional roles associated with OsPDF1B. Homozygous plants of pdf1b/pdf1b exhibited the phenotypes of chlorina and growth retardation. Histochemical analysis showed that the length of their mesophyll cells was increased 4- to 5-fold, resulting in a reduction in the total number of cells. Transmission electron microscopy analyses revealed that chloroplasts were severely damaged and mitochondria appeared to be mildly altered in the pdf1b mutants. Expression of genes encoded in ...Continue Reading

References

May 1, 1992·Plant Molecular Biology·M Sugiura
Nov 14, 1997·Neuroreport·P GuptanM M Panicker
Nov 12, 1998·Trends in Biochemical Sciences·F JeanmouginT J Gibson
Apr 6, 2000·Plant & Cell Physiology·Y Y YamamotoX W Deng
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·J S JeonG An
Dec 29, 2000·Nature·M SalanoubatUNKNOWN Kazusa DNA Research Institute
Dec 6, 2001·Journal of Molecular Biology·A SereroT Meinnel
Jul 18, 2002·The Plant Journal : for Cell and Molecular Biology·Thomas A BeardsleeLori A Allison
Sep 18, 2002·Archives of Biochemistry and Biophysics·Lynnette M A DirkRobert L Houtz
Dec 14, 2002·Plant Physiology·Dong-Hoon JeongGynheung An
Dec 31, 2002·The EMBO Journal·Carmela GiglioneThierry Meinnel
Feb 8, 2003·Science·Robert M LarkinJoanne Chory
May 30, 2003·Plant & Cell Physiology·Ki-Hong JungGynheung An
Oct 9, 2003·The Journal of Biological Chemistry·Alexandre SereroThierry Meinnel
Nov 11, 2003·Trends in Plant Science·Agepati S Raghavendra, Kollipara Padmasree
Nov 13, 2003·Current Opinion in Plant Biology·Marcio C Silva-Filho
Jun 16, 2004·Cellular and Molecular Life Sciences : CMLS·C GiglioneT Meinnel
May 3, 2005·Journal of Experimental Botany·Vidal FeyThomas Pfannschmidt
Jun 14, 2005·Plant Molecular Biology·Sichul LeeGynheung An
Aug 16, 2005·Nature·UNKNOWN International Rice Genome Sequencing Project
Sep 6, 2005·The Plant Cell·Ki-Hong JungGynheung An
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Dong-Hoon JeongGynheung An
Apr 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hongbo GaoKatherine W Osteryoung
Jun 15, 2006·Annual Review of Genetics·Zhengchang Liu, Ronald A Butow
Jul 27, 2007·Molecular Microbiology·Nabile Bouzaidi-TialiAndré Schneider

❮ Previous
Next ❯

Citations

Feb 14, 2009·Molecular Genetics and Genomics : MGG·Carolina V MorganteMarcio C Silva-Filho
Apr 21, 2009·Plant Physiology·Sichul LeeGynheung An
Jun 18, 2009·Expert Opinion on Therapeutic Targets·Anshika SharmaSadhna Sharma
Nov 12, 2015·Biochimica Et Biophysica Acta·Adina BreimanCarmela Giglione
Jan 13, 2011·The Plant Journal : for Cell and Molecular Biology·Jong-Jin ParkGynheung An
Jun 16, 2010·The Plant Journal : for Cell and Molecular Biology·Wiebke ApelRalph Bock
Jun 12, 2012·Biochimica Et Biophysica Acta·Christopher Carrie, Ian Small
Aug 18, 2012·Plant Signaling & Behavior·Mohammad Amin OmidbakhshfardBernd Mueller-Roeber
May 19, 2012·International Journal of Molecular Sciences·Chang-Cai LiuChuan-Ping Yang
Jan 2, 2021·Trends in Plant Science·Carmela Giglione, Thierry Meinnel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.