PMID: 9526498Apr 4, 1998Paper

Rice proteins that bind single-stranded G-rich telomere DNA

Plant Molecular Biology
J H KimI K Chung

Abstract

In this work, we have identified and characterized proteins in rice nuclear extracts that specifically bind the single-stranded G-rich telomere sequence. Three types of specific DNA-protein complexes (I, II, and III) were identified by gel retardation assays using synthetic telomere substrates consisting of two or more single-stranded TTTAGGG repeats and rice nuclear extracts. Since each complex has a unique biochemical property and differs in electrophoretic mobility, at least three different proteins interact with the G-rich telomere sequences. These proteins are called rice G-rich telomere binding protein (RGBP) and none of them show binding affinity to double-stranded telomere repeats or single-stranded C-rich sequence. Changing one or two G's to C's in the TTTAGGG repeats abolishes binding activity. RGBPs have a greatly reduced affinity for human and Tetrahymena telomeric sequence and do not efficiently bind the cognate G-rich telomere RNA sequence UUUAGGG. Like other telomere binding proteins, RGBPs are resistant to high salt concentrations. RNase sensitivity of the DNA-protein interaction. In this assay, we observed a novel complex (complex III) in gel retardation assays which did not alter the mobilities or the band int...Continue Reading

Citations

Jul 27, 2005·Molecular & Cellular Proteomics : MCP·Paula CasatiVirginia Walbot
May 17, 2000·The Journal of Biological Chemistry·E Y YuI K Chung
Nov 8, 2001·DNA Sequence : the Journal of DNA Sequencing and Mapping·A KatoM Ozaki
Apr 3, 2004·Genome Génome / Conseil National De Recherches Canada·Petra SchrumpfováJirí Fajkus
Mar 30, 2001·The Journal of Biological Chemistry·C M ChenC H Ho
Feb 21, 2009·The Plant Journal : for Cell and Molecular Biology·Eugene V ShakirovDorothy E Shippen
Jul 26, 2016·Frontiers in Plant Science·Petra Procházková SchrumpfováJiří Fajkus
Apr 6, 2004·Plant Physiology and Biochemistry : PPB·Yoshinori HirataShingo Sakai
Mar 27, 2007·Biochimica Et Biophysica Acta·Barbara Zellinger, Karel Riha

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