Rice starch, amylopectin, and amylose: molecular weight and solubility in dimethyl sulfoxide-based solvents

Journal of Agricultural and Food Chemistry
Fang ZhongCharles F Shoemaker

Abstract

Dimethyl sulfoxide (DMSO), with either 50 mM LiBr, 10% water, or both, was used as solvent for multi-angle laser-light scattering (MALLS) batch mode analysis of rice starch, and amylopectin and amylose weight-average molecular weight (Mw). DMSO/50 mM LiBr was a better solvent for these measurements than was DMSO/10% water, based on this solvent's ability to dissolve starch and to reduce the size of starch aggregates. Starch concentration decreased and amylose:amylopectin ratio increased when starch suspended in DMSO was centrifuged or filtered prior to size-exclusion chromatography (SEC)-MALLS analysis. A higher amylose:amylopectin ratio made starch more soluble, and the higher this ratio, the lower the Mw of eluted amylopectin. For SEC analysis of Mw, fractions of starch amylopectin and amylose dispersed in DMSO-based solvents yielded better results than starch dispersed directly into the solvents, because dispersion of these fractions decreased starch aggregation. When these two starch components were fractionated and then dissolved separately in DMSO/50 mM LiBr, the Mw of dispersed amylopectin ranged from 40 to 50 million, and that of amylose was ca. 3 million, whereas starch from three rice varieties of varying amylose cont...Continue Reading

References

Nov 17, 2001·Biomacromolecules·S RadostaW Vorwerg

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Citations

Nov 26, 2010·Analytical and Bioanalytical Chemistry·Robert G Gilbert
Oct 19, 2011·Archaea : an International Microbiological Journal·Annarita PoliBarbara Nicolaus
Apr 23, 2014·Carbohydrate Polymers·Chutarat Kowittaya, Namfone Lumdubwong
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Dec 20, 2008·Journal of Agricultural and Food Chemistry·Kietsuda Luengwilai, Diane M Beckles

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