Rickettsia peacockii, an endosymbiont of Dermacentor andersoni, does not elicit or inhibit humoral immune responses from immunocompetent D. andersoni or Ixodes scapularis cell lines

Developmental and Comparative Immunology
Joshua T MattilaT J Kurtti

Abstract

Ixodes scapularis and Dermacentor andersoni cell lines were stimulated with heat-killed Escherichia coli and Micrococcus luteus to investigate whether infection by Rickettsia peacockii, an endosymbiont of D. andersoni, modifies humoral immune responses. Radial diffusion assays, western blotting, flow cytometry, and quantitative reverse-transcription PCR were used to determine if expression of bacteriolytic peptides, including lysozyme and defensin, was upregulated by bacterial stimulation or infection with R. peacockii. The I. scapularis line IDE12 upregulated expression of lysozyme and defensin following stimulation. The D. andersoni cell line DAE15 also expressed defensin and lysozyme, but only lysozyme was upregulated by bacterial stimulation. R. peacockii infection alone, or in cells stimulated with bacteria, did not modify defensin or lysozyme expression in either cell line. These results suggest tick endosymbionts may avoid recognition by the tick immune system, and infection may not affect humoral immune responses to bacteria not normally associated with ticks.

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Citations

Dec 7, 2007·Proceedings. Biological Sciences·Eleanor R Haine
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Ivo M B FrancischettiJose M C Ribeiro
Nov 13, 2012·Ticks and Tick-borne Diseases·M Pilar AlberdiLesley Bell-Sakyi
Apr 15, 2008·Insect Biochemistry and Molecular Biology·Eliane EstevesSirlei Daffre
Mar 28, 2018·Infection and Immunity·Emma K HarrisKevin R Macaluso
Nov 19, 2020·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Hugh LefcortChristy C Andrade
Jun 25, 2021·Trends in Parasitology·Hanna J Laukaitis, Kevin R Macaluso

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