PMID: 374996Jan 16, 1979Paper

Rifampicin supersensitivity of rho strains of E. coli, and suppression by sur mutation

Molecular & General Genetics : MGG
S K Guterman, C L Howitt

Abstract

Escherichia coli strains with mutations rho-115, rho-ts15, rho-101 (psu-1) or rho-102 (psu-2) are more sensitive ("supersensitive") to rifampicin than isogenic parent strains, as measured by growth rate in broth and colony forming efficiency on solid media with 5, 10, or 20 microgram of rifampicin per ml. There is no change in sensitivity of rho mutants to the antibiotics penicillin, erythromycin, chloramphenicol, or the detergent desoxycholate. The rho-101 or rho-102 mutations confer rifampicin supersensitivity at 32 degrees C but not 42 degrees C. Mutants of a rho-115 strain that have lost polarity suppression can be isolated by selection for rifampicin resistance. This phenotype, Sur, is not due to reversion of the original rho gene mutation but to a second mutation perhaps in the gene for rho protein or the gene for the beta subunit of RNA polymerase. One class of Sur mutation, occurring in rho-115 cells isolated as resistant to 20 microgram of rifampicin per ml, is co-transducible with the marker ilv, and the gene order is rbs-ilv-sur-38. A model suggested by this map position is that the mutations rho-115 and sur-38 define the domain of rho protein which interacts with the beta subunit of RNA polymerase.

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A DasS Adhya
May 25, 1977·Journal of Molecular Biology·L P GuarenteJ Beckwith
Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
May 15, 1976·Journal of Molecular Biology·L J Korn, C Yanofsky
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y Nakamura, T Yura
Jun 4, 1975·Science·K BertrandC Yanofsky
Feb 4, 1974·Biochemical and Biophysical Research Communications·H Yang, G Zubay
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C Lowery-Goldhammer, J P Richardson
Jun 1, 1973·Journal of Bacteriology·S K Guterman
Jun 25, 1973·Journal of Molecular Biology·E G Minkley, D Pribnow
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·M H Malamy
Dec 20, 1969·Nature·J W Roberts
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C P Georgopoulos
Mar 28, 1972·Journal of Molecular Biology·A Ghysen, M Pironio

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·H KungD H Calhoun
Sep 1, 1986·Molecular & General Genetics : MGG·J S FasslerI Tessman
Jan 1, 1983·Molecular & General Genetics : MGG·P PutnokyA Kondorosi
Jan 1, 1980·Molecular & General Genetics : MGG·S K GutermanG Singer
Jan 1, 1981·Molecular & General Genetics : MGG·R B Kent, S K Guterman
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R LatheE K Bautz
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R B Kent, S K Guterman
Dec 5, 2012·Genes & Development·Jason M PetersRobert Landick
Apr 1, 1992·Molecular Microbiology·I B Dicker, S Seetharam
Jan 15, 1985·European Journal of Biochemistry·D O Nwankwo, S K Guterman
Apr 20, 1985·Journal of Molecular Biology·S BarikA Das
Jan 1, 1984·Molecular & General Genetics : MGG·D H CalhounS K Guterman
Jan 1, 1980·Molecular & General Genetics : MGG·Y N Zograff, A L Gintsburg
May 9, 2021·Applied Microbiology and Biotechnology·Tomás G VillaAngeles Sánchez-Pérez
Jan 1, 1984·Journal of Bacteriology·C YoungD H Figurski
Feb 1, 1985·Journal of Bacteriology·J S FasslerE S Tessman
Jan 1, 1985·Journal of Bacteriology·D H CalhounH F Kung
Jul 1, 1984·Journal of Bacteriology·S AksoyC Squires

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