Rift Valley fever virus L segment: correction of the sequence and possible functional role of newly identified regions conserved in RNA-dependent polymerases

The Journal of General Virology
R MüllerM Bouloy

Abstract

The sequence of Rift Valley fever virus L segment that we published in a previous paper was erroneous in the 3'-terminal region of the antigenomic RNA molecule. Here, we have shown that the L segment is in fact 6404 nucleotides long and encodes a polypeptide of 237.7K in the viral complementary sense. Sequence comparisons performed between the RNA-dependent RNA polymerases of 22 negative-stranded RNA viruses revealed the existence of two novel regions located at the amino termini of the proteins and conserved only in the polymerases of bunya- and arenaviruses. In the region conserved in all RNA-dependent polymerases, corresponding to the so-called 'polymerase module', we identified a new motif, designated premotif A, common to all RNA-dependent polymerases, as well as amino acids located in the region between motifs preA and A which are strictly conserved for segmented negative-stranded RNA viruses. Using the recently released coordinates of human immunodeficiency virus reverse transcriptase and the alignment between all RNA-dependent polymerases in the 'polymerase module', we have determined the position of the conserved residues in these polymerases and discuss their possible functions in light of the available structural inf...Continue Reading

Citations

Aug 19, 2007·Archives of Virology·C CarromeuA M Ventura
Sep 25, 2009·Archives of Virology·Toufic ElbeainoGiovanni P Martelli
Sep 18, 2009·Virus Genes·Stacey L WattsJames E Maruniak
Oct 2, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Simon ViethStephan Günther
Oct 9, 2002·Virus Research·John D MeissnerStephen C St Jeor
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Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·James F PapinTilahun D Yilma
Jun 12, 2010·The Journal of Biological Chemistry·Stéphane BoivinDarren J Hart
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Oct 9, 2009·Journal of Virology·Aya Zamoto-NiikuraShinji Makino

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