Right ventricular myocardial biomarkers in human heart failure

Journal of Cardiac Failure
Thomas G di SalvoAnna Hemnes

Abstract

Right ventricular (RV) dysfunction contributes to mortality in chronic heart failure (HF). However, the molecular mechanisms of RV failure remain poorly understood, and RV myocardial biomarkers have yet to be developed. We performed RNA sequencing (RNA-seq) on 22 explanted human HF RVs and 5 unused donor human heart RVs (DON RV) and compared results to those recently reported from 16 explanted human LVs We used Bowtie-Tophat for transcript alignment and transcriptome assembly, DESeq for identification of differentially expressed genes (DEGs) and Ingenuity for exploration of gene ontologies. In the HF RV, RNA-seq identified 130,790 total RNA transcripts including 13,272 protein coding genes, 10,831 long non-coding RNA genes and 8,605 pseudogenes. There were 800-1000 DEGs between DON and HF RV comparison groups with differences concentrated in cytoskeletal, basement membrane, extracellular matrix (ECM), inflammatory mediator, hemostasis, membrane transport and transcription factor genes, lncRNAs and pseudogenes. In an unbiased approach, the top 10 DEGs SERPINA3, SERPINA5, LCN6, LCN10, STEAP4, AKR1C1, STAC2, SPARCL1, VSIG4 and F8 exhibited no overlap in read counts between DON and HF RVs, high sensitivities, specificities, predict...Continue Reading

References

Dec 12, 2001·Circulation·P RazeghiH Taegtmeyer
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Dec 31, 2010·Nature Reviews. Genetics·Fatih Ozsolak, Patrice M Milos
May 31, 2011·Nature Methods·Manuel GarberCole Trapnell
Aug 11, 2011·Circulation. Cardiovascular Genetics·Andreas S BarthGordon F Tomaselli
Feb 14, 2012·Human Molecular Genetics·Noboru J SakabeMarcelo A Nobrega
Mar 28, 2012·Nature Reviews. Cardiology·Tariq AhmadChristopher O'Connor
May 3, 2012·Cardiology Clinics·Louis J Dell'Italia
Sep 27, 2012·Progress in Cardiovascular Diseases·Maria G Karas, Jorge R Kizer
Oct 30, 2012·Cell·Jakob LovénRichard A Young
Nov 7, 2012·Pulmonary Circulation·Evan L BrittainTom Disalvo
Jan 3, 2013·The Journal of Clinical Investigation·Jop H van BerloJeffery D Molkentin
Mar 7, 2013·Nature Structural & Molecular Biology·Tim R Mercer, John S Mattick
Mar 12, 2013·Molecular Cell·Leah R SabinGregory J Hannon
Mar 19, 2013·Cell·Ulrich BraunschweigBenjamin J Blencowe
Apr 19, 2013·Nature Reviews. Genetics·Bonnie BergerMona Singh
May 7, 2013·Current Cardiology Reports·Punam ChowdhuryAlan Maisel
Jun 19, 2013·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Amresh RainaPaul R Forfia
Jul 25, 2013·Chest·Vasile ForisHorst Olschewski

❮ Previous
Next ❯

Citations

Jun 19, 2016·Journal of Translational Medicine·Simona GrecoFabio Martelli
Sep 26, 2017·Clinical and Translational Science·Ana Caroline C SáJulie A Johnson
Apr 14, 2018·Circulation·Marvin A KonstamUNKNOWN American Heart Association Council on Clinical Cardiology; Council on Cardiovascular Disease in the Young; and Council on Ca
Feb 21, 2018·Circulation·Wouter C MeijersRudolf A de Boer
May 24, 2019·Circulation Research·Jason G E ZeltDuncan J Stewart
Apr 26, 2019·JCI Insight·Christos TzimasEmily J Tsai
Oct 14, 2017·Annals of Medicine·Samira KalayiniaNejat Mahdieh
Aug 12, 2015·Current Opinion in Pediatrics·Sushma Reddy, Daniel Bernstein
Nov 3, 2020·Frontiers in Cardiovascular Medicine·Jonathan J EdwardsZoltan Arany
Mar 2, 2021·Frontiers in Molecular Biosciences·Yanyan QiSongtao An
Apr 3, 2021·The European Respiratory Journal·Aida Llucià-ValldeperasFrances Sarah de Man
Jan 26, 2018·Life Sciences·Jordan L WilliamsElisa A Bradley
Apr 10, 2021·The European Respiratory Journal·Soni Savai PullamsettiWerner Seeger
Jun 3, 2021·International Journal of Environmental Research and Public Health·Sek Ying ChairWai Tong Chien
Nov 6, 2021·Scandinavian Cardiovascular Journal : SCJ·Uzair AnsariIbrahim Akin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

© 2022 Meta ULC. All rights reserved