Apr 15, 2020

A modular microscale granuloma model for immune-microenvironment signaling studies in vitro

BioRxiv : the Preprint Server for Biology
S. B. BerryAshleigh Brooks Theberge

Abstract

Tuberculosis (TB) is one of the most potent infectious diseases in the world, causing more deaths than any other single infectious agent. TB infection is caused by inhalation of Mycobacterium tuberculosis (Mtb) and subsequent phagocytosis and migration into the lung tissue by innate immune cells (e.g., alveolar macrophages, neutrophils, dendritic cells), resulting in the formation of a fused mass of immune cells known as the granuloma. Considered the pathological hallmark of TB, the granuloma is a complex microenvironment that is crucial for pathogen containment as well as pathogen survival. Disruption of the delicate granuloma microenvironment via numerous stimuli, such as variations in cytokine secretions, nutrient availability, and the makeup of immune cell population, can lead to an active infection. Herein, we present a novel in vitro model to examine the soluble factor signaling between a mycobacterial infection and its surrounding environment. Adapting a newly developed suspended microfluidic platform, known as Stacks, we established a modular microscale infection model containing human immune cells and a model mycobacterial strain that can easily integrate with different microenvironmental cues through simple spatial an...Continue Reading

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Mentioned in this Paper

Study
Muscle Rigidity
Silicone Gels
Specimen Type - Fibroblasts
Extracellular Matrix Constituent Secretion
Keratinocyte
Extracellular Matrix
Cell Differentiation Process
Intercellular Communication Process
Hydrogels

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