RipAY, a Plant Pathogen Effector Protein, Exhibits Robust γ-Glutamyl Cyclotransferase Activity When Stimulated by Eukaryotic Thioredoxins.

The Journal of Biological Chemistry
Shoko FujiwaraMitsuaki Tabuchi

Abstract

The plant pathogenic bacterium Ralstonia solanacearum injects more than 70 effector proteins (virulence factors) into the host plant cells via the needle-like structure of a type III secretion system. The type III secretion system effector proteins manipulate host regulatory networks to suppress defense responses with diverse molecular activities. Uncovering the molecular function of these effectors is essential for a mechanistic understanding of R. solanacearum pathogenicity. However, few of the effectors from R. solanacearum have been functionally characterized, and their plant targets remain largely unknown. Here, we show that the ChaC domain-containing effector RipAY/RSp1022 from R. solanacearum exhibits γ-glutamyl cyclotransferase (GGCT) activity to degrade the major intracellular redox buffer, glutathione. Heterologous expression of RipAY, but not other ChaC family proteins conserved in various organisms, caused growth inhibition of yeast Saccharomyces cerevisiae, and the intracellular glutathione level was decreased to ∼30% of the normal level following expression of RipAY in yeast. Although active site mutants of GGCT activity were non-toxic, the addition of glutathione did not reverse the toxicity, suggesting that the ...Continue Reading

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Citations

Aug 5, 2016·Frontiers in Cellular and Infection Microbiology·Crina M PopaMarc Valls
Feb 26, 2016·PLoS Pathogens·Crina PopaGuido Sessa
Jun 20, 2017·PLoS Pathogens·Teja ShidoreLindsay R Triplett
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May 26, 2017·Antioxidants & Redox Signaling·Anand Kumar Bachhawat, Amandeep Kaur
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Apr 2, 2021·Plant Physiology·Jade R Bleau, Steven H Spoel
Apr 22, 2021·Molecular Plant-microbe Interactions : MPMI·Ankita PandeyKee Hoon Sohn
Oct 18, 2021·The New Phytologist·Samuel EastmanMing Guo

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