RIPPS: A Plant Phenotyping System for Quantitative Evaluation of Growth Under Controlled Environmental Stress Conditions

Plant & Cell Physiology
Miki FujitaKazuo Shinozaki

Abstract

High-throughput and accurate measurements of plant traits facilitate identification of gene function. Along with recent advances in quantitative genomics, there is a growing need for precise quantification of multiple traits in plants. However, it is difficult continuously to quantify plant adaptive responses to environmental stress responses such as drought because multiple environmental factors are intricately involved in the phenotype. To solve this problem, we developed an automatic phenotyping system for evaluating the growth responses of individual Arabidopsis plants to a wide range of environmental conditions. The RIKEN Integrated Plant Phenotyping System (RIPPS) controls soil moisture for single plants by automatically weighing and watering 120 continuously rotating pots under controlled light, humidity and temperature growth conditions. RIPPS also records individual rosette size and expansion rate by photographing plants every 2 h. We used RIPPS to establish phenotype evaluation methods for Arabidopsis growth response and water use efficiency under various water conditions, and analyzed the involvement of ABA metabolism in determining water use efficiency. We also used RIPPS to analyze salinity tolerance in Arabidopsis...Continue Reading

References

May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·H ShiJ K Zhu
Nov 15, 2000·Trends in Plant Science·L Chaerle, D Van Der Straeten
Jun 6, 2002·The Plant Journal : for Cell and Molecular Biology·Sylvain MerlotJérôme Giraudat
Sep 21, 2004·Annual Review of Plant Biology·Maarten KoornneefDick Vreugdenhil
Apr 16, 2005·Current Opinion in Biotechnology·Kirsi-Marja Oksman-Caldentey, Kazuki Saito
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Paul E VersluesJian-Kang Zhu
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Taishi UmezawaKazuo Shinozaki
May 4, 2006·Annual Review of Plant Biology·Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
Nov 28, 2008·The Plant Journal : for Cell and Molecular Biology·Kaoru UranoKazuo Shinozaki
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Makoto TakanoTomoko Shinomura
Apr 7, 2010·Current Opinion in Biotechnology·Aleksandra Skirycz, Dirk Inzé
Mar 11, 2011·Nature Biotechnology·Aleksandra SkiryczDirk Inzé
Mar 5, 2013·The Plant Journal : for Cell and Molecular Biology·Sébastien TisnéOlivier Loudet
Mar 19, 2014·Current Opinion in Plant Biology·Christine Granier, Denis Vile
Jan 22, 2015·Plant Physiology·Pieter ClauwDirk Inzé
Nov 26, 2015·Current Opinion in Plant Biology·Shintaro MunemasaJulian I Schroeder
Jun 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhenyu YangErwin Grill
Sep 7, 2016·Plant Science : an International Journal of Experimental Plant Biology·Takashi KuromoriKazuo Shinozaki
Oct 8, 2016·Cell·Jian-Kang Zhu
Aug 9, 2017·Current Biology : CB·François TardieuMalcolm Bennett

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Citations

Oct 16, 2019·Journal of Experimental Botany·Justyna Jadwiga OlasFederico Apelt
May 12, 2020·Plant & Cell Physiology·Keiichi MochidaTakashi Hirayama
Jul 1, 2020·Plant & Cell Physiology·Keiichi MochidaTakashi Hirayama
Aug 28, 2020·Frontiers in Plant Science·Marni TausenStig Uggerhøj Andersen
Feb 11, 2020·Frontiers in Plant Science·Damiano MartignagoAna I Caño-Delgado
Jul 4, 2021·The Plant Journal : for Cell and Molecular Biology·Lirong XiangLie Tang
Dec 5, 2021·The Plant Journal : for Cell and Molecular Biology·Takashi KuromoriKazuo Shinozaki
Jan 21, 2022·Plant Molecular Biology·Kaoru UranoKazuo Shinozaki

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