PMID: 9539974Apr 16, 1998Paper

Risk assessment in cervical dysplasia patients by single cell gel electrophoresis assay: a study of DNA damage and repair

Mutation Research
A UdumudiY R Ahuja

Abstract

Precancerous lesions of cervix, commonly known as dysplasia, present a complex problem because of their biological behavior. Increased genetic instability, either inherent or induced by some external mutagen, is considered as a primary event or a predisposing factor to neoplastic transformation. The relationship between genetic instability and susceptibility towards cervical cancer was evaluated with the comet or single cell gel electrophoresis (SCGE) assay. Among precancerous individuals, genomic instability was observed in cervical epithelial cells and peripheral blood leukocytes. The mean basal DNA damage and mean susceptibility to DNA damage by the mutagen (MNNG) treatment increased whereas repair capacity decreased with progression of the disease in a stepwise manner. Inter and intra individual variability was maximum in cancerous group. Risk was estimated by giving a predictive value for each precancerous individual. In combination with morphological, biochemical, and cytogenetic parameters, the SCGE assay may serve as a novel tool to predict the fate of cervical dysplasia.

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Citations

Mar 30, 2013·Pathology Oncology Research : POR·Luis Orlando PérezCarlos Daniel Golijow
Jan 1, 2003·Cancer Detection and Prevention·Huifeng ZhangXifeng Wu
Oct 25, 2012·Journal of Biomedicine & Biotechnology·Elva I Cortés-GutiérrezRicardo M Cerda-Flores
Jul 16, 2014·T︠S︡itologii︠a︡ i genetika·E I Cortés-GutiérrezR M Cerda-Flores
Jan 9, 2007·Toxicology·Y R AhujaK Polasa
Jul 20, 2017·Drug and Chemical Toxicology·Tayyaba AliQaiser M Khan
Jul 17, 2010·Genetic Testing and Molecular Biomarkers·Mohan VasaviQurratulain Hasan
Dec 21, 2005·Carcinogenesis·Kristin L LockettJennifer J Hu
Sep 11, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·E I Cortés-GutiérrezJ Gosálvez
Sep 5, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jian GuXifeng Wu
Feb 25, 2021·Archives of Gynecology and Obstetrics·Elva I Cortés-GutiérrezJaime Gosálvez
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde

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